Counteranions in the Stimulation Solution Alter the Dynamics of Exocytosis Consistent with the Hofmeister Series

Xiulan He, Andrew G. Ewing

Research output: Contribution to journalArticlepeer-review

Abstract

We show that the Hofmeister series of ions can be used to explain the cellular changes in exocytosis observed by single-cell amperometry for different counteranions. The formation, expansion, and closing of the membrane fusion pore during exocytosis was found to be strongly dependent on the counteranion species in solution. With stimulation of chaotropic anions (e.g., ClO4-), the expansion and closing time of the fusion pore are longer, suggesting chaotropes can extend the duration of exocytosis compared with kosmotropic anions (e.g., Cl-). At a concentration of 30 mM, the two parameters (e.g., t1/2 and tfall) that define the duration of exocytosis vary with the Hofmeister series (Cl- < Br- < NO3- ≤ ClO4- < SCN-). More interestingly, fewer (e.g., Nfoot/Nevents) and smaller (e.g., Ifoot) prespike events are observed when chaotropes are counterions in the stimulation solution, and the values can be sorted by the reverse Hofmeister series (Cl- ≥ Br- > NO3- > ClO4- > SCN-). Based on ion specificity, an adsorption-repulsion mechanism, we suggest that the exocytotic Hofmeister series effect originates from a looser swelling lipid bilayer structure due to the adsorption and electrostatic repulsion of chaotropes on the hydrophobic portion of the membrane. Our results provide a chemical link between the Hofmeister series and the cellular process of neurotransmitter release via exocytosis and provide a better physical framework to understand this important phenomenon.

Original languageEnglish (US)
Pages (from-to)12591-12595
Number of pages5
JournalJournal of the American Chemical Society
Volume142
Issue number29
DOIs
StatePublished - Jul 22 2020

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Fingerprint Dive into the research topics of 'Counteranions in the Stimulation Solution Alter the Dynamics of Exocytosis Consistent with the Hofmeister Series'. Together they form a unique fingerprint.

Cite this