Cra-dependent transcriptional activation of the icd gene of Escherichia coli

Jean François Prost, Didier Nègre, Christelle Oudot, Katsuhiko Murakami, Akira Ishihama, Alain J. Cozzone, Jean Claude Cortay

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

The icd gene of Escherichia coli, encoding isocitrate dehydrogenase, was shown to be expressed from two different promoters: the previously identified icd P1 and a newly detected second promoter, icd P2, whose expression is positively regulated by the catabolite repressor-activator protein Cra, formerly called FruR. In each case, we determined the mRNA start site by primer extension analysis of in vivo transcripts and examined the interaction of the icd control region with either RNA polymerase or Cra. We observed that (i) the Cra factor binds to and activates transcription from a site centered at position -76.5 within the icd P2 promoter region and (ii) three particular mutations in the C-terminal end of the α subunit of RNA polymerase (L262A, R265A, and N268A) considerably diminish transcription initiating from the icd P2 promoter, as shown by in vitro experiments performed in the presence of mutant RNA polymerases carrying Ala substitutions.

Original languageEnglish (US)
Pages (from-to)893-898
Number of pages6
JournalJournal of bacteriology
Volume181
Issue number3
DOIs
StatePublished - Feb 1999

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Biology

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