Cranberry extract attenuates hepatic inflammation in high-fat-fed obese mice

Shannon L. Glisan, Caroline Ryan, Andrew P. Neilson, Joshua D. Lambert

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cranberry (Vaccinium macrocarpon) consumption has been associated with health beneficial effects. Nonalcoholic fatty liver disease (NAFLD) is a comorbidity of obesity. In the present study, we investigated the effect of a polyphenol-rich cranberry extract (CBE) on hepatic inflammation in high fat (HF)-fed obese C57BL/6J mice. Following dietary treatment with 0.8% CBE for 10 weeks, we observed no change in body weight or visceral fat mass in CBE-supplemented mice compared to HF-fed control mice. We did observe a significant decrease in plasma alanine aminotransferase (31%) and histological severity of NAFLD (33% decrease in area of involvement, 29% decrease in lipid droplet size) compared to HF-fed controls. Hepatic protein levels of tumor necrosis factor α and C-C chemokine ligand 2 were reduced by 28% and 19%, respectively, following CBE supplementation. CBE significantly decreased hepatic mRNA levels of toll-like receptor 4 (TLR4, 63%) and nuclear factor κB (NFκB, 24%), as well as a number of genes related to the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing 3 inflammasome. In conclusion, CBE reduced NAFLD and hepatic inflammation in HF-fed obese C57BL/6J mice. These effects appear to be related to mitigation of TLR4-NFκB related signaling; however, further studies into the underlying mechanisms of these hepatoprotective effects are needed.

Original languageEnglish (US)
Pages (from-to)60-66
Number of pages7
JournalJournal of Nutritional Biochemistry
Volume37
DOIs
StatePublished - Nov 1 2016

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Vaccinium macrocarpon
Obese Mice
Fats
Inflammation
Liver
Lymphotoxin-beta
Inflammasomes
CC Chemokines
Inbred C57BL Mouse
Toll-Like Receptor 4
Polyphenols
Alanine Transaminase
Leucine
Nucleotides
Genes
Body Weight Changes
Intra-Abdominal Fat
Health
Ligands
Lipids

All Science Journal Classification (ASJC) codes

  • Endocrinology, Diabetes and Metabolism
  • Biochemistry
  • Molecular Biology
  • Nutrition and Dietetics
  • Clinical Biochemistry

Cite this

Glisan, Shannon L. ; Ryan, Caroline ; Neilson, Andrew P. ; Lambert, Joshua D. / Cranberry extract attenuates hepatic inflammation in high-fat-fed obese mice. In: Journal of Nutritional Biochemistry. 2016 ; Vol. 37. pp. 60-66.
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abstract = "Cranberry (Vaccinium macrocarpon) consumption has been associated with health beneficial effects. Nonalcoholic fatty liver disease (NAFLD) is a comorbidity of obesity. In the present study, we investigated the effect of a polyphenol-rich cranberry extract (CBE) on hepatic inflammation in high fat (HF)-fed obese C57BL/6J mice. Following dietary treatment with 0.8{\%} CBE for 10 weeks, we observed no change in body weight or visceral fat mass in CBE-supplemented mice compared to HF-fed control mice. We did observe a significant decrease in plasma alanine aminotransferase (31{\%}) and histological severity of NAFLD (33{\%} decrease in area of involvement, 29{\%} decrease in lipid droplet size) compared to HF-fed controls. Hepatic protein levels of tumor necrosis factor α and C-C chemokine ligand 2 were reduced by 28{\%} and 19{\%}, respectively, following CBE supplementation. CBE significantly decreased hepatic mRNA levels of toll-like receptor 4 (TLR4, 63{\%}) and nuclear factor κB (NFκB, 24{\%}), as well as a number of genes related to the nucleotide-binding domain, leucine-rich-containing family, pyrin domain-containing 3 inflammasome. In conclusion, CBE reduced NAFLD and hepatic inflammation in HF-fed obese C57BL/6J mice. These effects appear to be related to mitigation of TLR4-NFκB related signaling; however, further studies into the underlying mechanisms of these hepatoprotective effects are needed.",
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Cranberry extract attenuates hepatic inflammation in high-fat-fed obese mice. / Glisan, Shannon L.; Ryan, Caroline; Neilson, Andrew P.; Lambert, Joshua D.

In: Journal of Nutritional Biochemistry, Vol. 37, 01.11.2016, p. 60-66.

Research output: Contribution to journalArticle

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