Creating addressable aqueous microcompartments above solid supported phospholipid bilayers using lithographically patterned poly(dimethylsiloxane) molds

Tinglu Yang, Eric E. Simanek, Paul Cremer

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

Herein we report the use of microcontact displacement (μCD) to generate addressable arrays of aqueous solutions above fluid lipid bilayers by bringing a patterned PDMS mold into conformal contact with a phospholipid membrane on a solid supported substrate. Epifluorescence microscopy established that the bilayer material was displaced in regions where contact between the mold and substrate was made. Photobleaching experiments confirmed that the bilayer sectors remained individually fluid but completely separated. The microcompartments created by μCD could be individually injected with aqueous solutions that remained sealed from their neighbors. This procedure was then exploited for screening a small library of aqueous- phase molecules for their ability to inhibit binding between surface-bound ligands and soluble protein receptors.

Original languageEnglish (US)
Pages (from-to)2587-2589
Number of pages3
JournalAnalytical chemistry
Volume72
Issue number11
DOIs
StatePublished - Jun 1 2000

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Fingerprint Dive into the research topics of 'Creating addressable aqueous microcompartments above solid supported phospholipid bilayers using lithographically patterned poly(dimethylsiloxane) molds'. Together they form a unique fingerprint.

  • Cite this