Creating fluid and air-stable solid supported lipid bilayers

Matthew A. Holden, Seung Yong Jung, Tinglu Yang, Edward T. Castellana, Paul S. Cremer

Research output: Contribution to journalArticle

77 Citations (Scopus)

Abstract

Solid supported lipid bilayers are rapidly delaminated when drawn through the air/water interface. We have discovered that a close packed monolayer of specifically bound protein prevents this process. The protection mechanism worked in two ways. First, when protein-protected bilayers were drawn through the air/water interface, a thin bulk water layer was visible over the entire bilayer region, thereby preventing air from contacting the surface. Second, a stream of nitrogen was used to remove all bulk water from a protected bilayer, which remained fully intact as determined by fluorescence microscopy. The condition of this dried bilayer was further probed by fluorescence recovery after photobleaching. It was found that lipids were not two-dimensionally mobile in dry air. However, when the bilayer was placed in a humid environment, 91% of the bleached fluorescence signal was recovered, indicating long-range two-dimensional mobility. The diffusion coefficient of lipids under humid conditions was an order of magnitude slower than the same bilayer under water. Protected bilayers could be rehydrated after drying, and their characteristic diffusion coefficient was reestablished. Insights into the mechanism of bilayer preservation were suggested.

Original languageEnglish (US)
Pages (from-to)6512-6513
Number of pages2
JournalJournal of the American Chemical Society
Volume126
Issue number21
DOIs
StatePublished - Jun 2 2004

Fingerprint

Lipid bilayers
Lipid Bilayers
Air
Fluids
Water
Lipids
Fluorescence
Fluorescence Recovery After Photobleaching
Proteins
Photobleaching
Fluorescence microscopy
Fluorescence Microscopy
Monolayers
Drying
Nitrogen
Recovery

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

Cite this

Holden, Matthew A. ; Jung, Seung Yong ; Yang, Tinglu ; Castellana, Edward T. ; Cremer, Paul S. / Creating fluid and air-stable solid supported lipid bilayers. In: Journal of the American Chemical Society. 2004 ; Vol. 126, No. 21. pp. 6512-6513.
@article{cd53e131beef4dd5a6cac6060f2bac33,
title = "Creating fluid and air-stable solid supported lipid bilayers",
abstract = "Solid supported lipid bilayers are rapidly delaminated when drawn through the air/water interface. We have discovered that a close packed monolayer of specifically bound protein prevents this process. The protection mechanism worked in two ways. First, when protein-protected bilayers were drawn through the air/water interface, a thin bulk water layer was visible over the entire bilayer region, thereby preventing air from contacting the surface. Second, a stream of nitrogen was used to remove all bulk water from a protected bilayer, which remained fully intact as determined by fluorescence microscopy. The condition of this dried bilayer was further probed by fluorescence recovery after photobleaching. It was found that lipids were not two-dimensionally mobile in dry air. However, when the bilayer was placed in a humid environment, 91{\%} of the bleached fluorescence signal was recovered, indicating long-range two-dimensional mobility. The diffusion coefficient of lipids under humid conditions was an order of magnitude slower than the same bilayer under water. Protected bilayers could be rehydrated after drying, and their characteristic diffusion coefficient was reestablished. Insights into the mechanism of bilayer preservation were suggested.",
author = "Holden, {Matthew A.} and Jung, {Seung Yong} and Tinglu Yang and Castellana, {Edward T.} and Cremer, {Paul S.}",
year = "2004",
month = "6",
day = "2",
doi = "10.1021/ja048504a",
language = "English (US)",
volume = "126",
pages = "6512--6513",
journal = "Journal of the American Chemical Society",
issn = "0002-7863",
publisher = "American Chemical Society",
number = "21",

}

Creating fluid and air-stable solid supported lipid bilayers. / Holden, Matthew A.; Jung, Seung Yong; Yang, Tinglu; Castellana, Edward T.; Cremer, Paul S.

In: Journal of the American Chemical Society, Vol. 126, No. 21, 02.06.2004, p. 6512-6513.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Creating fluid and air-stable solid supported lipid bilayers

AU - Holden, Matthew A.

AU - Jung, Seung Yong

AU - Yang, Tinglu

AU - Castellana, Edward T.

AU - Cremer, Paul S.

PY - 2004/6/2

Y1 - 2004/6/2

N2 - Solid supported lipid bilayers are rapidly delaminated when drawn through the air/water interface. We have discovered that a close packed monolayer of specifically bound protein prevents this process. The protection mechanism worked in two ways. First, when protein-protected bilayers were drawn through the air/water interface, a thin bulk water layer was visible over the entire bilayer region, thereby preventing air from contacting the surface. Second, a stream of nitrogen was used to remove all bulk water from a protected bilayer, which remained fully intact as determined by fluorescence microscopy. The condition of this dried bilayer was further probed by fluorescence recovery after photobleaching. It was found that lipids were not two-dimensionally mobile in dry air. However, when the bilayer was placed in a humid environment, 91% of the bleached fluorescence signal was recovered, indicating long-range two-dimensional mobility. The diffusion coefficient of lipids under humid conditions was an order of magnitude slower than the same bilayer under water. Protected bilayers could be rehydrated after drying, and their characteristic diffusion coefficient was reestablished. Insights into the mechanism of bilayer preservation were suggested.

AB - Solid supported lipid bilayers are rapidly delaminated when drawn through the air/water interface. We have discovered that a close packed monolayer of specifically bound protein prevents this process. The protection mechanism worked in two ways. First, when protein-protected bilayers were drawn through the air/water interface, a thin bulk water layer was visible over the entire bilayer region, thereby preventing air from contacting the surface. Second, a stream of nitrogen was used to remove all bulk water from a protected bilayer, which remained fully intact as determined by fluorescence microscopy. The condition of this dried bilayer was further probed by fluorescence recovery after photobleaching. It was found that lipids were not two-dimensionally mobile in dry air. However, when the bilayer was placed in a humid environment, 91% of the bleached fluorescence signal was recovered, indicating long-range two-dimensional mobility. The diffusion coefficient of lipids under humid conditions was an order of magnitude slower than the same bilayer under water. Protected bilayers could be rehydrated after drying, and their characteristic diffusion coefficient was reestablished. Insights into the mechanism of bilayer preservation were suggested.

UR - http://www.scopus.com/inward/record.url?scp=2542582259&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2542582259&partnerID=8YFLogxK

U2 - 10.1021/ja048504a

DO - 10.1021/ja048504a

M3 - Article

C2 - 15161253

AN - SCOPUS:2542582259

VL - 126

SP - 6512

EP - 6513

JO - Journal of the American Chemical Society

JF - Journal of the American Chemical Society

SN - 0002-7863

IS - 21

ER -