Crimped Nanofibrous Biomaterials Mimic Microstructure and Mechanics of Native Tissue and Alter Strain Transfer to Cells

Spencer E. Szczesny, Tristan P. Driscoll, Hsiao Yun Tseng, Pang Ching Liu, Su Jin Heo, Robert L. Mauck, Pen Hsiu G. Chao

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

To fully recapitulate tissue microstructure and mechanics, fiber crimping must exist within biomaterials used for tendon/ligament engineering. Existing crimped nanofibrous scaffolds produced via electrospinning are dense materials that prevent cellular infiltration into the scaffold interior. In this study, we used a sacrificial fiber population to increase the scaffold porosity and evaluated the effect on fiber crimping. We found that increasing scaffold porosity increased fiber crimping and ensured that the fibers properly uncrimped as the scaffolds were stretched by minimizing fiber-fiber interactions. Constitutive modeling demonstrated that the fiber uncrimping produced a nonlinear mechanical behavior similar to that of native tendon and ligament. Interestingly, fiber crimping altered strain transmission to the nuclei of cells seeded on the scaffolds, which may account for previously observed changes in gene expression. These crimped biomaterials are useful for developing functional fiber-reinforced tissues and for studying the effects of altered fiber crimping due to damage or degeneration.

Original languageEnglish (US)
Pages (from-to)2869-2876
Number of pages8
JournalACS Biomaterials Science and Engineering
Volume3
Issue number11
DOIs
StatePublished - Nov 13 2017

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Biocompatible Materials
Biomaterials
Mechanics
Tissue
Microstructure
Fibers
Scaffolds
Ligaments
Tendons
tebufenozide
Porosity
Electrospinning
Infiltration
Gene expression

All Science Journal Classification (ASJC) codes

  • Biomaterials
  • Biomedical Engineering

Cite this

Szczesny, Spencer E. ; Driscoll, Tristan P. ; Tseng, Hsiao Yun ; Liu, Pang Ching ; Heo, Su Jin ; Mauck, Robert L. ; Chao, Pen Hsiu G. / Crimped Nanofibrous Biomaterials Mimic Microstructure and Mechanics of Native Tissue and Alter Strain Transfer to Cells. In: ACS Biomaterials Science and Engineering. 2017 ; Vol. 3, No. 11. pp. 2869-2876.
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Crimped Nanofibrous Biomaterials Mimic Microstructure and Mechanics of Native Tissue and Alter Strain Transfer to Cells. / Szczesny, Spencer E.; Driscoll, Tristan P.; Tseng, Hsiao Yun; Liu, Pang Ching; Heo, Su Jin; Mauck, Robert L.; Chao, Pen Hsiu G.

In: ACS Biomaterials Science and Engineering, Vol. 3, No. 11, 13.11.2017, p. 2869-2876.

Research output: Contribution to journalArticle

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