CRISPR-based engineering of next-generation lactic acid bacteria

Claudio Hidalgo-Cantabrana, Sarah O'Flaherty, Rodolphe Barrangou

Research output: Contribution to journalReview articlepeer-review

27 Scopus citations

Abstract

The advent of CRISPR-based technologies has opened new avenues for the development of next-generation food microorganisms and probiotics with enhanced functionalities. Building off two decades of functional genomics studies unraveling the genetic basis for food fermentations and host–probiotic interactions, CRISPR technologies offer a wide range of opportunities to engineer commercially-relevant Lactobacillus and Bifidobacteria. Endogenous CRISPR–Cas systems can be repurposed to enhance gene expression or provide new features to improve host colonization and promote human health. Alternatively, engineered CRISPR–Cas systems can be harnessed to genetically modify probiotics and enhance their therapeutic potential to deliver vaccines or modulate the host immune response.

Original languageEnglish (US)
Pages (from-to)79-87
Number of pages9
JournalCurrent Opinion in Microbiology
Volume37
DOIs
StatePublished - Jun 1 2017

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)
  • Infectious Diseases

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