TY - JOUR
T1 - CRISPR-Cas Technologies and Applications in Food Bacteria
AU - Stout, Emily
AU - Klaenhammer, Todd
AU - Barrangou, Rodolphe
PY - 2017/2/28
Y1 - 2017/2/28
N2 - Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form adaptive immune systems that occur in many bacteria and most archaea. In addition to protecting bacteria from phages and other invasive mobile genetic elements, CRISPR-Cas molecular machines can be repurposed as tool kits for applications relevant to the food industry. A primary concern of the food industry has long been the proper management of food-related bacteria, with a focus on both enhancing the outcomes of beneficial microorganisms such as starter cultures and probiotics and limiting the presence of detrimental organisms such as pathogens and spoilage microorganisms. This review introduces CRISPR-Cas as a novel set of technologies to manage food bacteria and offers insights into CRISPR-Cas biology. It primarily focuses on the applications of CRISPR-Cas systems and tools in starter cultures and probiotics, encompassing strain-typing, phage resistance, plasmid vaccination, genome editing, and antimicrobial activity.
AB - Clustered regularly interspaced short palindromic repeats (CRISPRs) and CRISPR-associated (Cas) proteins form adaptive immune systems that occur in many bacteria and most archaea. In addition to protecting bacteria from phages and other invasive mobile genetic elements, CRISPR-Cas molecular machines can be repurposed as tool kits for applications relevant to the food industry. A primary concern of the food industry has long been the proper management of food-related bacteria, with a focus on both enhancing the outcomes of beneficial microorganisms such as starter cultures and probiotics and limiting the presence of detrimental organisms such as pathogens and spoilage microorganisms. This review introduces CRISPR-Cas as a novel set of technologies to manage food bacteria and offers insights into CRISPR-Cas biology. It primarily focuses on the applications of CRISPR-Cas systems and tools in starter cultures and probiotics, encompassing strain-typing, phage resistance, plasmid vaccination, genome editing, and antimicrobial activity.
UR - http://www.scopus.com/inward/record.url?scp=85014132510&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85014132510&partnerID=8YFLogxK
U2 - 10.1146/annurev-food-072816-024723
DO - 10.1146/annurev-food-072816-024723
M3 - Review article
C2 - 28245154
AN - SCOPUS:85014132510
VL - 8
SP - 413
EP - 437
JO - Annual review of food science and technology
JF - Annual review of food science and technology
SN - 1941-1413
ER -