TY - JOUR
T1 - CRISPR/Cas-Mediated Base Editing
T2 - Technical Considerations and Practical Applications
AU - Molla, Kutubuddin A.
AU - Yang, Yinong
N1 - Funding Information:
Kutubuddin Molla greatly acknowledges the USA-India Educational Foundation (USIEF), New Delhi, and the US Department of State for a Fulbright Nehru Post-Doctoral Fellowship . This work was supported by the National Science Foundation (NSF) Plant Genome Research Program (grant 1740874 ) and by United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) Hatch Act Appropriations under project PEN04659 and accession 1016432 to Yinong Yang.
Funding Information:
Kutubuddin Molla greatly acknowledges the USA-India Educational Foundation (USIEF), New Delhi, and the US Department of State for a Fulbright Nehru Post-Doctoral Fellowship. This work was supported by the National Science Foundation (NSF) Plant Genome Research Program (grant 1740874) and by United States Department of Agriculture (USDA) National Institute of Food and Agriculture (NIFA) Hatch Act Appropriations under project PEN04659 and accession 1016432 to Yinong Yang.
Publisher Copyright:
© 2019 Elsevier Ltd
PY - 2019/10
Y1 - 2019/10
N2 - Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
AB - Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
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U2 - 10.1016/j.tibtech.2019.03.008
DO - 10.1016/j.tibtech.2019.03.008
M3 - Review article
C2 - 30995964
AN - SCOPUS:85064170935
VL - 37
SP - 1121
EP - 1142
JO - Trends in Biotechnology
JF - Trends in Biotechnology
SN - 0167-7799
IS - 10
ER -