Abstract
Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1121-1142 |
| Number of pages | 22 |
| Journal | Trends in Biotechnology |
| Volume | 37 |
| Issue number | 10 |
| DOIs | |
| State | Published - Oct 1 2019 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
Cite this
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CRISPR/Cas-Mediated Base Editing : Technical Considerations and Practical Applications. / Molla, Kutubuddin A.; Yang, Yinong.
In: Trends in Biotechnology, Vol. 37, No. 10, 01.10.2019, p. 1121-1142.Research output: Contribution to journal › Review article
TY - JOUR
T1 - CRISPR/Cas-Mediated Base Editing
T2 - Technical Considerations and Practical Applications
AU - Molla, Kutubuddin A.
AU - Yang, Yinong
PY - 2019/10/1
Y1 - 2019/10/1
N2 - Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
AB - Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.
UR - http://www.scopus.com/inward/record.url?scp=85064170935&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85064170935&partnerID=8YFLogxK
U2 - 10.1016/j.tibtech.2019.03.008
DO - 10.1016/j.tibtech.2019.03.008
M3 - Review article
C2 - 30995964
AN - SCOPUS:85064170935
VL - 37
SP - 1121
EP - 1142
JO - Trends in Biotechnology
JF - Trends in Biotechnology
SN - 0167-7799
IS - 10
ER -