CRISPR/Cas-Mediated Base Editing: Technical Considerations and Practical Applications

Kutubuddin A. Molla, Yinong Yang

Research output: Contribution to journalReview article

6 Citations (Scopus)

Abstract

Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.

Original languageEnglish (US)
Pages (from-to)1121-1142
Number of pages22
JournalTrends in Biotechnology
Volume37
Issue number10
DOIs
StatePublished - Oct 1 2019

Fingerprint

Clustered Regularly Interspaced Short Palindromic Repeats
DNA
Genes
Agricultural Crops
Inborn Genetic Diseases
Double-Stranded DNA Breaks
Nucleotides
Agriculture
RNA
Medicine
Crops
Research
Gene Editing

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering

Cite this

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abstract = "Genome editing with CRISPR/Cas has rapidly gained popularity. Base editing, a new CRISPR/Cas-based approach, can precisely convert one nucleotide to another in DNA or RNA without inducing a double-strand DNA break (DSB). A combination of catalytically impaired nuclease variants with different deaminases has yielded diverse base-editing platforms that aim to address the key limitations such as specificity, protospacer adjacent motif (PAM) compatibility, editing window length, bystander editing, and sequence context preference. Because new base editors significantly reduce unintended editing in the genome, they hold great promise for treating genetic diseases and for developing superior agricultural crops. We review here the development of various base editors, assess their technical advantages and limitations, and discuss their broad applications in basic research, medicine, and agriculture.",
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CRISPR/Cas-Mediated Base Editing : Technical Considerations and Practical Applications. / Molla, Kutubuddin A.; Yang, Yinong.

In: Trends in Biotechnology, Vol. 37, No. 10, 01.10.2019, p. 1121-1142.

Research output: Contribution to journalReview article

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