Cross-Linking Chemistry of Tyramine-Modified Hyaluronan Hydrogels Alters Mesenchymal Stem Cell Early Attachment and Behavior

Claudia Loebel, Spencer Szczesny, Brian D. Cosgrove, Mauro Alini, Marcy Zenobi-Wong, Robert L. Mauck, David Eglin

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Given the significance of hydrogels as cell-instructive materials, it is important to understand how differences in their chemical and physical properties are able to direct cell fate. For example, it remains unclear how different hydrogel cross-linking chemistries and gelation mechanisms influence cell behavior. Here, we report on hyaluronan-tyramine (HA-Tyr) hydrogels prepared either with enzymatic cross-linking using horseradish peroxidase and H2O2 or with visible light (500 nm) triggered gelation. We demonstrate that when hydrogels are polymerized to equivalent Young's moduli, the specific cross-linking chemistry of HA-Tyr hydrogels can have a substantial impact on mesenchymal stem cell (MSC) behavior. MSCs cultured on HA-Tyr hydrogels exhibit increased cell spread areas on enzymatically formed substrates relative to photo-cross-linked matrices. While enzymatically formed hydrogels led to MSCs exhibiting greater cell focal adhesion length, MSCs cultured on the photo-cross-linked matrices exhibited smaller cell spread area and shorter focal adhesion length but generated increased traction stress. These findings highlight the importance of understanding hydrogel cross-linking chemistries when the role of biophysical cues in regulating stem cell fate is investigated.

Original languageEnglish (US)
Pages (from-to)855-864
Number of pages10
JournalBiomacromolecules
Volume18
Issue number3
DOIs
StatePublished - Mar 13 2017

Fingerprint

Tyramine
Hydrogels
Hyaluronic Acid
Stem cells
Hydrogel
Gelation
Adhesion
Horseradish Peroxidase
Chemical properties
Physical properties
Elastic moduli
Substrates

All Science Journal Classification (ASJC) codes

  • Bioengineering
  • Biomaterials
  • Polymers and Plastics
  • Materials Chemistry

Cite this

Loebel, Claudia ; Szczesny, Spencer ; Cosgrove, Brian D. ; Alini, Mauro ; Zenobi-Wong, Marcy ; Mauck, Robert L. ; Eglin, David. / Cross-Linking Chemistry of Tyramine-Modified Hyaluronan Hydrogels Alters Mesenchymal Stem Cell Early Attachment and Behavior. In: Biomacromolecules. 2017 ; Vol. 18, No. 3. pp. 855-864.
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Cross-Linking Chemistry of Tyramine-Modified Hyaluronan Hydrogels Alters Mesenchymal Stem Cell Early Attachment and Behavior. / Loebel, Claudia; Szczesny, Spencer; Cosgrove, Brian D.; Alini, Mauro; Zenobi-Wong, Marcy; Mauck, Robert L.; Eglin, David.

In: Biomacromolecules, Vol. 18, No. 3, 13.03.2017, p. 855-864.

Research output: Contribution to journalArticle

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