Cross-recognition of two middle T protein epitopes by immunodominant polyoma virus-specific CTL

Christopher S. Wilson, Janice M. Moser, John D. Altman, Peter E. Jensen, Aron Lukacher

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

We recently identified the immunodominant epitope for polyoma virus- specific CTL as the Dκ-associated peptide MT389-397 derived from the middle T (MT) viral oncoprotein. Another Dκ-restricted peptide corresponding to residues 236-244 of MT was recognized by nearly all MT389-397- reactive CTL clones, but required concentrations at least 2 logs higher to sensitize syngeneic target cells for lysis. Except for identity at the three putative Dκ-peptide anchor residues, MT236-244 shares no homology with MT389-397. Using a novel curopium-based class I MHC-peptide binding immunoassay, we determined that MT236-244 bound Dκ 2-3 logs less well than MT389-397. Infection with a mutant polyoma virus whose MT is truncated just before the MT389-397 epitope or immunization with MT389-397 or MT236-244 peptides elicited CTL that recognized both MT389-397 and MT236-244. Importantly, infection with a polyoma virus lacking MT389-397 and mutated in an MT236-244 Dκ anchor position induced polyoma virus-specific CTL recognizing neither MT389-397 nor MT236-244 epitopes. Despite predominant usage of the Vβ6 gene segment, MT389-397/MT236-244 cross-reactive CTL clones possess diverse complementarity-determining region 3β domains; this is functionally reflected in their heterogeneous recognition patterns of alanine-monosubstituted MT389-397 peptides. Using Dκ/MT389- 397 tetramers, we directly visualized MT236-244 peptide-induced TCR down-modulation of virtually all MT389-397-specific CD8+ T cells freshly explanted from polyoma-infected mice, suggesting that a single TcR recognizes both Dκ-restricted epitopes. The availability of immunodominant epitope-specific CTL capable of recognizing a second epitope in MT, a viral protein essential for tumorigenesis, may serve to amplify the CTL response to the immunodominant epitope and prevent the emergence of immunodominant epitope-loss viruses and virus-induced tumors.

Original languageEnglish (US)
Pages (from-to)3933-3941
Number of pages9
JournalJournal of Immunology
Volume162
Issue number7
StatePublished - Apr 1 1999

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Immunodominant Epitopes
Polyomavirus
Peptides
Epitopes
Proteins
Clone Cells
Complementarity Determining Regions
Oncogenic Viruses
Oncogene Proteins
Viral Proteins
Infection
Immunoassay
Alanine
Immunization
Carcinogenesis
Viruses
T-Lymphocytes
Genes

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Wilson, C. S., Moser, J. M., Altman, J. D., Jensen, P. E., & Lukacher, A. (1999). Cross-recognition of two middle T protein epitopes by immunodominant polyoma virus-specific CTL. Journal of Immunology, 162(7), 3933-3941.
Wilson, Christopher S. ; Moser, Janice M. ; Altman, John D. ; Jensen, Peter E. ; Lukacher, Aron. / Cross-recognition of two middle T protein epitopes by immunodominant polyoma virus-specific CTL. In: Journal of Immunology. 1999 ; Vol. 162, No. 7. pp. 3933-3941.
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abstract = "We recently identified the immunodominant epitope for polyoma virus- specific CTL as the Dκ-associated peptide MT389-397 derived from the middle T (MT) viral oncoprotein. Another Dκ-restricted peptide corresponding to residues 236-244 of MT was recognized by nearly all MT389-397- reactive CTL clones, but required concentrations at least 2 logs higher to sensitize syngeneic target cells for lysis. Except for identity at the three putative Dκ-peptide anchor residues, MT236-244 shares no homology with MT389-397. Using a novel curopium-based class I MHC-peptide binding immunoassay, we determined that MT236-244 bound Dκ 2-3 logs less well than MT389-397. Infection with a mutant polyoma virus whose MT is truncated just before the MT389-397 epitope or immunization with MT389-397 or MT236-244 peptides elicited CTL that recognized both MT389-397 and MT236-244. Importantly, infection with a polyoma virus lacking MT389-397 and mutated in an MT236-244 Dκ anchor position induced polyoma virus-specific CTL recognizing neither MT389-397 nor MT236-244 epitopes. Despite predominant usage of the Vβ6 gene segment, MT389-397/MT236-244 cross-reactive CTL clones possess diverse complementarity-determining region 3β domains; this is functionally reflected in their heterogeneous recognition patterns of alanine-monosubstituted MT389-397 peptides. Using Dκ/MT389- 397 tetramers, we directly visualized MT236-244 peptide-induced TCR down-modulation of virtually all MT389-397-specific CD8+ T cells freshly explanted from polyoma-infected mice, suggesting that a single TcR recognizes both Dκ-restricted epitopes. The availability of immunodominant epitope-specific CTL capable of recognizing a second epitope in MT, a viral protein essential for tumorigenesis, may serve to amplify the CTL response to the immunodominant epitope and prevent the emergence of immunodominant epitope-loss viruses and virus-induced tumors.",
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Wilson, CS, Moser, JM, Altman, JD, Jensen, PE & Lukacher, A 1999, 'Cross-recognition of two middle T protein epitopes by immunodominant polyoma virus-specific CTL', Journal of Immunology, vol. 162, no. 7, pp. 3933-3941.

Cross-recognition of two middle T protein epitopes by immunodominant polyoma virus-specific CTL. / Wilson, Christopher S.; Moser, Janice M.; Altman, John D.; Jensen, Peter E.; Lukacher, Aron.

In: Journal of Immunology, Vol. 162, No. 7, 01.04.1999, p. 3933-3941.

Research output: Contribution to journalArticle

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