Cross Sectional Study and Risk Factors Analysis of Francisella tularensis in Soil Samples in Punjab Province of Pakistan

Javed Muhammad, Masood Rabbani, Muhammad Zubair Shabbir, Khushi Muhammad, Muhammad Taslim Ghori, Haroon Rashid Chaudhry, Zia Ul Hassnain, Tariq Jamil, Tariq Abbas, Muhammad Hamid Chaudhry, Muhammad Haisem-Ur-Rasool, Muhammad Asad Ali, Muhammad Nisar, Girish Soorapp Kirimanjeswara, Bhushan M. Jayarao

Research output: Contribution to journalArticle

Abstract

Tularemia is an endemic zoonotic disease in many parts of the world including Asia. A cross-sectional study was conducted to determine genome-based prevalence of Francisella tularensis (Ft) in soil, assess an association between its occurrence in soil and likely predictors i.e., macro and micro-nutrients and several categorical variables, and determine seroconversion in small and large ruminants. The study included a total of 2,280 soil samples representing 456 villages in eight districts of the Punjab Province of Pakistan followed by an analysis of serum antibodies in 707 ruminants. The genome of Ft was detected in 3.25% (n = 74, 95% CI: 2.60-4.06) of soil samples. Soluble salts (OR: 1.276, 95% CI: 1.043-1.562, p = 0.015), Ni (OR: 2.910, 95%CI: 0.795-10.644, p = 0.106), Mn (OR:0.733, 95% CI:0.565-0.951, p = 0.019), Zn (OR: 4.922, 95% CI:0.929-26.064, p = 0.061) and nutrients clustered together as PC-1 (OR: 4.76, 95% CI: 2.37-9.54, p = 0.000) and PC-3 (OR: 0.357, 95% CI: 0.640, p = 0.001) were found to have a positive association for the presence of Ft in soil. The odds of occurrence of Ft DNA in soil were higher at locations close to a water source, including canals, streams or drains, [χ2 = 6.7, OR = 1.19, 95% CI:1.05-3.09, p = 0.004] as well as places where animals were present [χ2 = 4.09, OR = 2.06, 95% CI: 1.05-4.05, p = 0.02]. The seroconversion was detected in 6.22% (n = 44, 95% CI: 4.67-8.25) of domestic animals. An occurrence of Ft over a wide geographical region indicates its expansion to enzootic range, and demonstrates the need for further investigation among potential disease reservoirs and at-risk populations, such as farmers and veterinarians.

Original languageEnglish (US)
Article number089
JournalFrontiers in Cellular and Infection Microbiology
Volume9
Issue numberAPR
DOIs
StatePublished - Jan 1 2019

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Francisella tularensis
Pakistan
Statistical Factor Analysis
Soil
Cross-Sectional Studies
Ruminants
Disease Reservoirs
Genome
Tularemia
Food
Endemic Diseases
Veterinarians
Domestic Animals
Zoonoses
Salts
Water
Antibodies
DNA
Serum

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Muhammad, Javed ; Rabbani, Masood ; Shabbir, Muhammad Zubair ; Muhammad, Khushi ; Ghori, Muhammad Taslim ; Chaudhry, Haroon Rashid ; Hassnain, Zia Ul ; Jamil, Tariq ; Abbas, Tariq ; Chaudhry, Muhammad Hamid ; Haisem-Ur-Rasool, Muhammad ; Ali, Muhammad Asad ; Nisar, Muhammad ; Kirimanjeswara, Girish Soorapp ; Jayarao, Bhushan M. / Cross Sectional Study and Risk Factors Analysis of Francisella tularensis in Soil Samples in Punjab Province of Pakistan. In: Frontiers in Cellular and Infection Microbiology. 2019 ; Vol. 9, No. APR.
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abstract = "Tularemia is an endemic zoonotic disease in many parts of the world including Asia. A cross-sectional study was conducted to determine genome-based prevalence of Francisella tularensis (Ft) in soil, assess an association between its occurrence in soil and likely predictors i.e., macro and micro-nutrients and several categorical variables, and determine seroconversion in small and large ruminants. The study included a total of 2,280 soil samples representing 456 villages in eight districts of the Punjab Province of Pakistan followed by an analysis of serum antibodies in 707 ruminants. The genome of Ft was detected in 3.25{\%} (n = 74, 95{\%} CI: 2.60-4.06) of soil samples. Soluble salts (OR: 1.276, 95{\%} CI: 1.043-1.562, p = 0.015), Ni (OR: 2.910, 95{\%}CI: 0.795-10.644, p = 0.106), Mn (OR:0.733, 95{\%} CI:0.565-0.951, p = 0.019), Zn (OR: 4.922, 95{\%} CI:0.929-26.064, p = 0.061) and nutrients clustered together as PC-1 (OR: 4.76, 95{\%} CI: 2.37-9.54, p = 0.000) and PC-3 (OR: 0.357, 95{\%} CI: 0.640, p = 0.001) were found to have a positive association for the presence of Ft in soil. The odds of occurrence of Ft DNA in soil were higher at locations close to a water source, including canals, streams or drains, [χ2 = 6.7, OR = 1.19, 95{\%} CI:1.05-3.09, p = 0.004] as well as places where animals were present [χ2 = 4.09, OR = 2.06, 95{\%} CI: 1.05-4.05, p = 0.02]. The seroconversion was detected in 6.22{\%} (n = 44, 95{\%} CI: 4.67-8.25) of domestic animals. An occurrence of Ft over a wide geographical region indicates its expansion to enzootic range, and demonstrates the need for further investigation among potential disease reservoirs and at-risk populations, such as farmers and veterinarians.",
author = "Javed Muhammad and Masood Rabbani and Shabbir, {Muhammad Zubair} and Khushi Muhammad and Ghori, {Muhammad Taslim} and Chaudhry, {Haroon Rashid} and Hassnain, {Zia Ul} and Tariq Jamil and Tariq Abbas and Chaudhry, {Muhammad Hamid} and Muhammad Haisem-Ur-Rasool and Ali, {Muhammad Asad} and Muhammad Nisar and Kirimanjeswara, {Girish Soorapp} and Jayarao, {Bhushan M.}",
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Muhammad, J, Rabbani, M, Shabbir, MZ, Muhammad, K, Ghori, MT, Chaudhry, HR, Hassnain, ZU, Jamil, T, Abbas, T, Chaudhry, MH, Haisem-Ur-Rasool, M, Ali, MA, Nisar, M, Kirimanjeswara, GS & Jayarao, BM 2019, 'Cross Sectional Study and Risk Factors Analysis of Francisella tularensis in Soil Samples in Punjab Province of Pakistan', Frontiers in Cellular and Infection Microbiology, vol. 9, no. APR, 089. https://doi.org/10.3389/fcimb.2019.00089

Cross Sectional Study and Risk Factors Analysis of Francisella tularensis in Soil Samples in Punjab Province of Pakistan. / Muhammad, Javed; Rabbani, Masood; Shabbir, Muhammad Zubair; Muhammad, Khushi; Ghori, Muhammad Taslim; Chaudhry, Haroon Rashid; Hassnain, Zia Ul; Jamil, Tariq; Abbas, Tariq; Chaudhry, Muhammad Hamid; Haisem-Ur-Rasool, Muhammad; Ali, Muhammad Asad; Nisar, Muhammad; Kirimanjeswara, Girish Soorapp; Jayarao, Bhushan M.

In: Frontiers in Cellular and Infection Microbiology, Vol. 9, No. APR, 089, 01.01.2019.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cross Sectional Study and Risk Factors Analysis of Francisella tularensis in Soil Samples in Punjab Province of Pakistan

AU - Muhammad, Javed

AU - Rabbani, Masood

AU - Shabbir, Muhammad Zubair

AU - Muhammad, Khushi

AU - Ghori, Muhammad Taslim

AU - Chaudhry, Haroon Rashid

AU - Hassnain, Zia Ul

AU - Jamil, Tariq

AU - Abbas, Tariq

AU - Chaudhry, Muhammad Hamid

AU - Haisem-Ur-Rasool, Muhammad

AU - Ali, Muhammad Asad

AU - Nisar, Muhammad

AU - Kirimanjeswara, Girish Soorapp

AU - Jayarao, Bhushan M.

PY - 2019/1/1

Y1 - 2019/1/1

N2 - Tularemia is an endemic zoonotic disease in many parts of the world including Asia. A cross-sectional study was conducted to determine genome-based prevalence of Francisella tularensis (Ft) in soil, assess an association between its occurrence in soil and likely predictors i.e., macro and micro-nutrients and several categorical variables, and determine seroconversion in small and large ruminants. The study included a total of 2,280 soil samples representing 456 villages in eight districts of the Punjab Province of Pakistan followed by an analysis of serum antibodies in 707 ruminants. The genome of Ft was detected in 3.25% (n = 74, 95% CI: 2.60-4.06) of soil samples. Soluble salts (OR: 1.276, 95% CI: 1.043-1.562, p = 0.015), Ni (OR: 2.910, 95%CI: 0.795-10.644, p = 0.106), Mn (OR:0.733, 95% CI:0.565-0.951, p = 0.019), Zn (OR: 4.922, 95% CI:0.929-26.064, p = 0.061) and nutrients clustered together as PC-1 (OR: 4.76, 95% CI: 2.37-9.54, p = 0.000) and PC-3 (OR: 0.357, 95% CI: 0.640, p = 0.001) were found to have a positive association for the presence of Ft in soil. The odds of occurrence of Ft DNA in soil were higher at locations close to a water source, including canals, streams or drains, [χ2 = 6.7, OR = 1.19, 95% CI:1.05-3.09, p = 0.004] as well as places where animals were present [χ2 = 4.09, OR = 2.06, 95% CI: 1.05-4.05, p = 0.02]. The seroconversion was detected in 6.22% (n = 44, 95% CI: 4.67-8.25) of domestic animals. An occurrence of Ft over a wide geographical region indicates its expansion to enzootic range, and demonstrates the need for further investigation among potential disease reservoirs and at-risk populations, such as farmers and veterinarians.

AB - Tularemia is an endemic zoonotic disease in many parts of the world including Asia. A cross-sectional study was conducted to determine genome-based prevalence of Francisella tularensis (Ft) in soil, assess an association between its occurrence in soil and likely predictors i.e., macro and micro-nutrients and several categorical variables, and determine seroconversion in small and large ruminants. The study included a total of 2,280 soil samples representing 456 villages in eight districts of the Punjab Province of Pakistan followed by an analysis of serum antibodies in 707 ruminants. The genome of Ft was detected in 3.25% (n = 74, 95% CI: 2.60-4.06) of soil samples. Soluble salts (OR: 1.276, 95% CI: 1.043-1.562, p = 0.015), Ni (OR: 2.910, 95%CI: 0.795-10.644, p = 0.106), Mn (OR:0.733, 95% CI:0.565-0.951, p = 0.019), Zn (OR: 4.922, 95% CI:0.929-26.064, p = 0.061) and nutrients clustered together as PC-1 (OR: 4.76, 95% CI: 2.37-9.54, p = 0.000) and PC-3 (OR: 0.357, 95% CI: 0.640, p = 0.001) were found to have a positive association for the presence of Ft in soil. The odds of occurrence of Ft DNA in soil were higher at locations close to a water source, including canals, streams or drains, [χ2 = 6.7, OR = 1.19, 95% CI:1.05-3.09, p = 0.004] as well as places where animals were present [χ2 = 4.09, OR = 2.06, 95% CI: 1.05-4.05, p = 0.02]. The seroconversion was detected in 6.22% (n = 44, 95% CI: 4.67-8.25) of domestic animals. An occurrence of Ft over a wide geographical region indicates its expansion to enzootic range, and demonstrates the need for further investigation among potential disease reservoirs and at-risk populations, such as farmers and veterinarians.

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