Cross-species transmission of honey bee viruses in associated arthropods

Abby L. Levitt, Rajwinder Singh, Diana Lynn Cox-foster, Edwin George Rajotte, Kelli Hoover, Nancy M. Ostiguy, Edward C. Holmes

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non- Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees - black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non- Apis species and active replication of Israeli acute paralysis virus in one non- Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.

Original languageEnglish (US)
Pages (from-to)232-240
Number of pages9
JournalVirus Research
Volume176
Issue number1-2
DOIs
StatePublished - Sep 1 2013

Fingerprint

Honey
Arthropods
Bees
Viruses
RNA Viruses
Paralysis
Dicistroviridae
Ecology
Ecosystem

All Science Journal Classification (ASJC) codes

  • Cancer Research
  • Virology
  • Infectious Diseases

Cite this

Levitt, A. L., Singh, R., Cox-foster, D. L., Rajotte, E. G., Hoover, K., Ostiguy, N. M., & Holmes, E. C. (2013). Cross-species transmission of honey bee viruses in associated arthropods. Virus Research, 176(1-2), 232-240. https://doi.org/10.1016/j.virusres.2013.06.013
Levitt, Abby L. ; Singh, Rajwinder ; Cox-foster, Diana Lynn ; Rajotte, Edwin George ; Hoover, Kelli ; Ostiguy, Nancy M. ; Holmes, Edward C. / Cross-species transmission of honey bee viruses in associated arthropods. In: Virus Research. 2013 ; Vol. 176, No. 1-2. pp. 232-240.
@article{a1b8ce7120084588ab17398633dd1703,
title = "Cross-species transmission of honey bee viruses in associated arthropods",
abstract = "There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non- Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees - black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non- Apis species and active replication of Israeli acute paralysis virus in one non- Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.",
author = "Levitt, {Abby L.} and Rajwinder Singh and Cox-foster, {Diana Lynn} and Rajotte, {Edwin George} and Kelli Hoover and Ostiguy, {Nancy M.} and Holmes, {Edward C.}",
year = "2013",
month = "9",
day = "1",
doi = "10.1016/j.virusres.2013.06.013",
language = "English (US)",
volume = "176",
pages = "232--240",
journal = "Virus Research",
issn = "0168-1702",
publisher = "Elsevier",
number = "1-2",

}

Levitt, AL, Singh, R, Cox-foster, DL, Rajotte, EG, Hoover, K, Ostiguy, NM & Holmes, EC 2013, 'Cross-species transmission of honey bee viruses in associated arthropods', Virus Research, vol. 176, no. 1-2, pp. 232-240. https://doi.org/10.1016/j.virusres.2013.06.013

Cross-species transmission of honey bee viruses in associated arthropods. / Levitt, Abby L.; Singh, Rajwinder; Cox-foster, Diana Lynn; Rajotte, Edwin George; Hoover, Kelli; Ostiguy, Nancy M.; Holmes, Edward C.

In: Virus Research, Vol. 176, No. 1-2, 01.09.2013, p. 232-240.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cross-species transmission of honey bee viruses in associated arthropods

AU - Levitt, Abby L.

AU - Singh, Rajwinder

AU - Cox-foster, Diana Lynn

AU - Rajotte, Edwin George

AU - Hoover, Kelli

AU - Ostiguy, Nancy M.

AU - Holmes, Edward C.

PY - 2013/9/1

Y1 - 2013/9/1

N2 - There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non- Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees - black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non- Apis species and active replication of Israeli acute paralysis virus in one non- Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.

AB - There are a number of RNA virus pathogens that represent a serious threat to the health of managed honey bees (Apis mellifera). That some of these viruses are also found in the broader pollinator community suggests the wider environmental spread of these viruses, with the potential for a broader impact on ecosystems. Studies on the ecology and evolution of these viruses in the arthropod community as a whole may therefore provide important insights into these potential impacts. We examined managed A. mellifera colonies, nearby non- Apis hymenopteran pollinators, and other associated arthropods for the presence of five commonly occurring picorna-like RNA viruses of honey bees - black queen cell virus, deformed wing virus, Israeli acute paralysis virus, Kashmir bee virus and sacbrood virus. Notably, we observed their presence in several arthropod species. Additionally, detection of negative-strand RNA using strand-specific RT-PCR assays for deformed wing virus and Israeli acute paralysis virus suggests active replication of deformed wing virus in at least six non- Apis species and active replication of Israeli acute paralysis virus in one non- Apis species. Phylogenetic analysis of deformed wing virus also revealed that this virus is freely disseminating across the species sampled in this study. In sum, our study indicates that these viruses are not specific to the pollinator community and that other arthropod species have the potential to be involved in disease transmission in pollinator populations.

UR - http://www.scopus.com/inward/record.url?scp=84881373347&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84881373347&partnerID=8YFLogxK

U2 - 10.1016/j.virusres.2013.06.013

DO - 10.1016/j.virusres.2013.06.013

M3 - Article

C2 - 23845302

AN - SCOPUS:84881373347

VL - 176

SP - 232

EP - 240

JO - Virus Research

JF - Virus Research

SN - 0168-1702

IS - 1-2

ER -