Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

Jean-Paul Armache, Alexander Jarasch, Andreas M. Anger, Elizabeth Villa, Thomas Becker, Shashi Bhushan, Fabrice Jossinet, Michael Habeck, Gülcin Dindar, Sibylle Franckenberg, Viter Marquez, Thorsten Mielke, Michael Thomm, Otto Berninghausen, Birgitta Beatrix, Johannes Söding, Eric Westhof, Daniel N. Wilson, Roland Beckmann

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

Original languageEnglish (US)
Pages (from-to)19748-19753
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume107
Issue number46
DOIs
StatePublished - Nov 16 2010

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Ribosomes
Protein Biosynthesis
Genetic Code
Cryoelectron Microscopy
Bacterial Structures
Ribonucleoproteins
Triticum
Saccharomyces cerevisiae
X-Rays
Peptides
Proteins

All Science Journal Classification (ASJC) codes

  • General

Cite this

Armache, Jean-Paul ; Jarasch, Alexander ; Anger, Andreas M. ; Villa, Elizabeth ; Becker, Thomas ; Bhushan, Shashi ; Jossinet, Fabrice ; Habeck, Michael ; Dindar, Gülcin ; Franckenberg, Sibylle ; Marquez, Viter ; Mielke, Thorsten ; Thomm, Michael ; Berninghausen, Otto ; Beatrix, Birgitta ; Söding, Johannes ; Westhof, Eric ; Wilson, Daniel N. ; Beckmann, Roland. / Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution. In: Proceedings of the National Academy of Sciences of the United States of America. 2010 ; Vol. 107, No. 46. pp. 19748-19753.
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Armache, J-P, Jarasch, A, Anger, AM, Villa, E, Becker, T, Bhushan, S, Jossinet, F, Habeck, M, Dindar, G, Franckenberg, S, Marquez, V, Mielke, T, Thomm, M, Berninghausen, O, Beatrix, B, Söding, J, Westhof, E, Wilson, DN & Beckmann, R 2010, 'Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution', Proceedings of the National Academy of Sciences of the United States of America, vol. 107, no. 46, pp. 19748-19753. https://doi.org/10.1073/pnas.1009999107

Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution. / Armache, Jean-Paul; Jarasch, Alexander; Anger, Andreas M.; Villa, Elizabeth; Becker, Thomas; Bhushan, Shashi; Jossinet, Fabrice; Habeck, Michael; Dindar, Gülcin; Franckenberg, Sibylle; Marquez, Viter; Mielke, Thorsten; Thomm, Michael; Berninghausen, Otto; Beatrix, Birgitta; Söding, Johannes; Westhof, Eric; Wilson, Daniel N.; Beckmann, Roland.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, No. 46, 16.11.2010, p. 19748-19753.

Research output: Contribution to journalArticle

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T1 - Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-Å resolution

AU - Armache, Jean-Paul

AU - Jarasch, Alexander

AU - Anger, Andreas M.

AU - Villa, Elizabeth

AU - Becker, Thomas

AU - Bhushan, Shashi

AU - Jossinet, Fabrice

AU - Habeck, Michael

AU - Dindar, Gülcin

AU - Franckenberg, Sibylle

AU - Marquez, Viter

AU - Mielke, Thorsten

AU - Thomm, Michael

AU - Berninghausen, Otto

AU - Beatrix, Birgitta

AU - Söding, Johannes

AU - Westhof, Eric

AU - Wilson, Daniel N.

AU - Beckmann, Roland

PY - 2010/11/16

Y1 - 2010/11/16

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AB - Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-Å resolution. This map, together with a 6.1-Å map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model ∼98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.

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