Abstract
Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.
| Original language | English (US) |
|---|---|
| Title of host publication | Advances in Virus Research |
| Editors | Félix A. Rey |
| Publisher | Academic Press Inc. |
| Pages | 73-91 |
| Number of pages | 19 |
| ISBN (Print) | 9780128184561 |
| DOIs | |
| State | Published - 2019 |
Publication series
| Name | Advances in Virus Research |
|---|---|
| Volume | 105 |
| ISSN (Print) | 0065-3527 |
| ISSN (Electronic) | 1557-8399 |
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All Science Journal Classification (ASJC) codes
- Virology
- Infectious Diseases
Cite this
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CryoEM reconstruction approaches to resolve asymmetric features. / Goetschius, Daniel J.; Lee, Hyunwook; Hafenstein, Susan.
Advances in Virus Research. ed. / Félix A. Rey. Academic Press Inc., 2019. p. 73-91 (Advances in Virus Research; Vol. 105).Research output: Chapter in Book/Report/Conference proceeding › Chapter
TY - CHAP
T1 - CryoEM reconstruction approaches to resolve asymmetric features
AU - Goetschius, Daniel J.
AU - Lee, Hyunwook
AU - Hafenstein, Susan
PY - 2019
Y1 - 2019
N2 - Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.
AB - Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.
UR - http://www.scopus.com/inward/record.url?scp=85071886582&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85071886582&partnerID=8YFLogxK
U2 - 10.1016/bs.aivir.2019.07.007
DO - 10.1016/bs.aivir.2019.07.007
M3 - Chapter
C2 - 31522709
AN - SCOPUS:85071886582
SN - 9780128184561
T3 - Advances in Virus Research
SP - 73
EP - 91
BT - Advances in Virus Research
A2 - Rey, Félix A.
PB - Academic Press Inc.
ER -