@inbook{bd3e078aaeea4d6c8ebdc569c256e43a,
title = "CryoEM reconstruction approaches to resolve asymmetric features",
abstract = "Although icosahedral viruses have highly symmetrical capsid features, asymmetric structural elements are also present since the genome and minor structural proteins are usually incorporated without adhering to icosahedral symmetry. Besides this inherent asymmetry, interactions with the host during the virus life cycle are also asymmetric. However, until recently it was impossible to resolve high resolution asymmetric features during single-particle cryoEM image processing. This review summarizes the current approaches that can be used to visualize asymmetric structural features. We have included examples of advanced structural strategies developed to reveal unique features and asymmetry in icosahedral viruses.",
author = "Goetschius, {Daniel J.} and Hyunwook Lee and Susan Hafenstein",
note = "Funding Information: This work was funded in part by the Pennsylvania Department of Health CURE funds, Huck Institutes at The Pennsylvania State University, and by the Office of the Director, National Institutes of Health, under Award Numbers 1 R01 AI107121-01 (SH). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Publisher Copyright: {\textcopyright} 2019 Elsevier Inc.",
year = "2019",
doi = "10.1016/bs.aivir.2019.07.007",
language = "English (US)",
isbn = "9780128184561",
series = "Advances in Virus Research",
publisher = "Academic Press Inc.",
pages = "73--91",
editor = "Rey, {F{\'e}lix A.}",
booktitle = "Complementary Strategies to Understand Virus Structure and Function",
address = "United States",
}
