Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila

Prabha P. Iyer, Sarah H. Lawrence, Kelvin B. Luther, Kanagalaghatta R. Rajashankar, Hemant P. Yennawar, James Gregory Ferry, Hermann Schindelin

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Phosphotransacetylase (Pta) [EC 2.3.1.8] is ubiquitous in the carbon assimilation and energy-yielding pathways in anaerobic prokaryotes where it catalyzes the reversible transfer of the acetyl group from acetyl phosphate to CoA forming acetyl CoA and inorganic phosphate. The crystal structure of Pta from the methane-producing archaeon Methanosarcina thermophila, representing the first crystal structure of any Pta, was determined by multiwavelength anomalous diffraction at 2.7 Å resolution. In solution and in the crystal, the enzyme forms a homodimer. Each monomer consists of two α/β domains with a cleft along the domain boundary, which presumably contains the substrate binding sites. Comparison of the four monomers present in the asymmetric unit indicates substantial variations in the relative orientation of the two domains and the structure of the putative active site cleft. A search for structural homologs revealed the NADP+-dependent isocitrate and isopropylmalate dehydrogenases as the only homologs with a similar two-domain architecture.

Original languageEnglish (US)
Pages (from-to)559-567
Number of pages9
JournalStructure
Volume12
Issue number4
DOIs
StatePublished - Apr 1 2004

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Phosphate Acetyltransferase
Methanosarcina
Archaea
Isocitrate Dehydrogenase
Acetyl Coenzyme A
Methane
Coenzyme A
NADP
Catalytic Domain
Carbon
Phosphates
Binding Sites
Enzymes

All Science Journal Classification (ASJC) codes

  • Structural Biology
  • Molecular Biology

Cite this

Iyer, Prabha P. ; Lawrence, Sarah H. ; Luther, Kelvin B. ; Rajashankar, Kanagalaghatta R. ; Yennawar, Hemant P. ; Ferry, James Gregory ; Schindelin, Hermann. / Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila. In: Structure. 2004 ; Vol. 12, No. 4. pp. 559-567.
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Crystal structure of phosphotransacetylase from the methanogenic archaeon Methanosarcina thermophila. / Iyer, Prabha P.; Lawrence, Sarah H.; Luther, Kelvin B.; Rajashankar, Kanagalaghatta R.; Yennawar, Hemant P.; Ferry, James Gregory; Schindelin, Hermann.

In: Structure, Vol. 12, No. 4, 01.04.2004, p. 559-567.

Research output: Contribution to journalArticle

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AU - Iyer, Prabha P.

AU - Lawrence, Sarah H.

AU - Luther, Kelvin B.

AU - Rajashankar, Kanagalaghatta R.

AU - Yennawar, Hemant P.

AU - Ferry, James Gregory

AU - Schindelin, Hermann

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