Abstract
Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 808-810 |
| Number of pages | 3 |
| Journal | Acta Crystallographica Section F: Structural Biology and Crystallization Communications |
| Volume | 66 |
| Issue number | 7 |
| DOIs | |
| State | Published - Jul 15 2010 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Genetics
- Condensed Matter Physics
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Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase. / Anandan, Anandhi; Vallet, Corinne; Coyle, Travis; Moustafa, Ibrahim M.; Vrielink, Alice.
In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 7, 15.07.2010, p. 808-810.Research output: Contribution to journal › Article
TY - JOUR
T1 - Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase
AU - Anandan, Anandhi
AU - Vallet, Corinne
AU - Coyle, Travis
AU - Moustafa, Ibrahim M.
AU - Vrielink, Alice
PY - 2010/7/15
Y1 - 2010/7/15
N2 - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.
AB - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.
UR - http://www.scopus.com/inward/record.url?scp=77954442386&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=77954442386&partnerID=8YFLogxK
U2 - 10.1107/S1744309110017185
DO - 10.1107/S1744309110017185
M3 - Article
C2 - 20606279
AN - SCOPUS:77954442386
VL - 66
SP - 808
EP - 810
JO - Acta Crystallographica Section F:Structural Biology Communications
JF - Acta Crystallographica Section F:Structural Biology Communications
SN - 1744-3091
IS - 7
ER -