Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Anandhi Anandan, Corinne Vallet, Travis Coyle, Ibrahim M. Moustafa, Alice Vrielink

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

Original languageEnglish (US)
Pages (from-to)808-810
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number7
DOIs
StatePublished - Jul 15 2010

Fingerprint

Penicillin Amidase
Crystallization
amidase
Diffraction
crystallization
Crystals
glycols
polyethylenes
Pseudomonas
Dehydration
diffraction
Glycerol
crystals
pseudomonas
glycerols
dehydration
acids

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

Cite this

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Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase. / Anandan, Anandhi; Vallet, Corinne; Coyle, Travis; Moustafa, Ibrahim M.; Vrielink, Alice.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 7, 15.07.2010, p. 808-810.

Research output: Contribution to journalArticle

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