Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Anandhi Anandan; Corinne Vallet; Travis Coyle; Ibrahim M. Moustafa; Alice Vrielink

doi:10.1107/S1744309110017185

Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Anandhi Anandan, Corinne Vallet, Travis Coyle, Ibrahim M. Moustafa, Alice Vrielink

Eberly College of Science

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

Original language	English (US)
Pages (from-to)	808-810
Number of pages	3
Journal	Acta Crystallographica Section F: Structural Biology and Crystallization Communications
Volume	66
Issue number	7
DOIs	https://doi.org/10.1107/S1744309110017185
State	Published - 2010

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Genetics
Condensed Matter Physics

Access to Document

10.1107/S1744309110017185

Cite this

@article{0f139bf0383c4ccc92193f3dee16f7ea,

title = "Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase",

abstract = "Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 {\AA} diffraction data set could be obtained.",

author = "Anandhi Anandan and Corinne Vallet and Travis Coyle and Moustafa, {Ibrahim M.} and Alice Vrielink",

year = "2010",

doi = "10.1107/S1744309110017185",

language = "English (US)",

volume = "66",

pages = "808--810",

journal = "Acta Crystallographica Section F:Structural Biology Communications",

issn = "1744-3091",

publisher = "John Wiley and Sons Ltd",

number = "7",

}

Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase. / Anandan, Anandhi; Vallet, Corinne; Coyle, Travis; Moustafa, Ibrahim M.; Vrielink, Alice.

In: Acta Crystallographica Section F: Structural Biology and Crystallization Communications, Vol. 66, No. 7, 2010, p. 808-810.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

AU - Anandan, Anandhi

AU - Vallet, Corinne

AU - Coyle, Travis

AU - Moustafa, Ibrahim M.

AU - Vrielink, Alice

PY - 2010

Y1 - 2010

N2 - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

AB - Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

UR - http://www.scopus.com/inward/record.url?scp=77954442386&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954442386&partnerID=8YFLogxK

U2 - 10.1107/S1744309110017185

DO - 10.1107/S1744309110017185

M3 - Article

C2 - 20606279

AN - SCOPUS:77954442386

VL - 66

SP - 808

EP - 810

JO - Acta Crystallographica Section F:Structural Biology Communications

JF - Acta Crystallographica Section F:Structural Biology Communications

SN - 1744-3091

IS - 7

ER -

Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Abstract

All Science Journal Classification (ASJC) codes

Access to Document

Other files and links

Fingerprint

Cite this