Crystallization and preliminary diffraction analysis of an engineered cephalosporin acylase

Anandhi Anandan, Corinne Vallet, Travis Coyle, Ibrahim M. Moustafa, Alice Vrielink

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Crystallization conditions are reported for an engineered cephalosporin acylase based on the sequence of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas strain N176. Initial crystals were grown using polyethylene glycol as a crystallizing agent; however, these crystals diffracted poorly and exhibited high mosaicity. A dehydration procedure in which crystals were transferred to a solution containing a higher concentration of polyethylene glycol as well as glycerol improved the diffraction quality such that a 1.57 Å diffraction data set could be obtained.

Original languageEnglish (US)
Pages (from-to)808-810
Number of pages3
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume66
Issue number7
DOIs
StatePublished - 2010

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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