Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle

Che Yen Wang, Naoyuki Miyazaki, Tetsuo Yamashita, Akifumi Higashiura, Atsushi Nakagawa, Tian Cheng Li, Naokazu Takeda, Li Xing, Erik Hjalmarsson, Claes Friberg, Der Ming Liou, Yen Jen Sung, Tomitake Tsukihara, Yoshiharu Matsuura, Tatsuo Miyamura, R. Holland Cheng

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Abstract

Hepatitis E virus (HEV) accounts for the majority of enterically transmitted hepatitis infections worldwide. Currently, there is no specific treatment for or vaccine against HEV. The major structural protein is derived from open reading frame (ORF) 2 of the viral genome. A potential oral vaccine is provided by the virus-like particles formed by a protein construct of partial ORF3 protein (residue 70-123) fused to the N-terminus of the ORF2 protein (residues 112-608). Single crystals obtained by the hanging-drop vapour-diffusion method at 293 K diffract X-rays to 8.3 Å resolution. The crystals belong to space group P212121, with unit-cell parameters a = 337, b = 343, c = 346 Å, α = β = γ = 90°, and contain one particle per asymmetric unit.

Original languageEnglish (US)
Pages (from-to)318-322
Number of pages5
JournalActa Crystallographica Section F: Structural Biology and Crystallization Communications
Volume64
Issue number4
DOIs
StatePublished - Mar 29 2008

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Condensed Matter Physics

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    Wang, C. Y., Miyazaki, N., Yamashita, T., Higashiura, A., Nakagawa, A., Li, T. C., Takeda, N., Xing, L., Hjalmarsson, E., Friberg, C., Liou, D. M., Sung, Y. J., Tsukihara, T., Matsuura, Y., Miyamura, T., & Cheng, R. H. (2008). Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis e virus-like particle. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 64(4), 318-322. https://doi.org/10.1107/S1744309108007197