Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley, Lori C. Zeringue, Richard D. Kidd, B. Tracy Nixon, Gregory K. Farber

Research output: Contribution to journalArticle

Abstract

The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 Å3 Da-1.

Original languageEnglish (US)
Pages (from-to)1416-1418
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number6 PART II
Publication statusPublished - Dec 1 1998

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Structural Biology

Cite this