The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 Å3 Da-1.
|Original language||English (US)|
|Number of pages||3|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Issue number||6 PART II|
|Publication status||Published - Dec 1 1998|
All Science Journal Classification (ASJC) codes
- Structural Biology