Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain

Mark Staley, Lori C. Zeringue, Richard D. Kidd, B. Tracy Nixon, Gregory K. Farber

Research output: Contribution to journalArticle

Abstract

The Rhizobium meliloti DctD two-component receiver domain was expressed in Escherichia coli and purified to homogeneity. Crystals were obtained using the hanging-drop vapor-diffusion geometry with ammonium phosphate as the precipitant. The crystals diffract to 2.3 Å and exhibit the symmetry of space group I222 or I212121. The unit-cell dimensions are a = 59.0, b = 58.6 and c = 169.8 Å. The asymmetric unit contains a dimer and the crystals have a Vm of 2.16 Å3 Da-1.

Original languageEnglish (US)
Pages (from-to)1416-1418
Number of pages3
JournalActa Crystallographica Section D: Biological Crystallography
Volume54
Issue number6 PART II
StatePublished - 1998

Fingerprint

Sinorhizobium meliloti
Crystallization
receivers
X-Rays
crystallization
Escherichia coli
X rays
Crystals
ammonium phosphates
crystals
x rays
Crystal symmetry
Escherichia
Dimers
homogeneity
Vapors
dimers
vapors
Geometry
symmetry

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Biophysics
  • Clinical Biochemistry
  • Structural Biology
  • Condensed Matter Physics

Cite this

Staley, Mark ; Zeringue, Lori C. ; Kidd, Richard D. ; Nixon, B. Tracy ; Farber, Gregory K. / Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain. In: Acta Crystallographica Section D: Biological Crystallography. 1998 ; Vol. 54, No. 6 PART II. pp. 1416-1418.
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Crystallization and preliminary X-ray studies of the Rhizobium meliloti DctD two-component receiver domain. / Staley, Mark; Zeringue, Lori C.; Kidd, Richard D.; Nixon, B. Tracy; Farber, Gregory K.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 54, No. 6 PART II, 1998, p. 1416-1418.

Research output: Contribution to journalArticle

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AU - Farber, Gregory K.

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