Crystallization of RNA/protein complexes

Maria Garber, George Gongadze, Vladimir Meshcheryakov, Oleg Nikonov, Alexey Nikulin, Anna Perederina, Wolfgang Piendl, Alexander Serganov, Svetlana Tishchenko

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Abstract

Different complexes of ribosomal proteins with specific rRNA fragments have been crystallized and studied by our group during the last six years. There are several factors important for successful crystallization of RNA/protein complexes, among them: length and content of RNA fragments, homogeneity of RNA and protein preparations, stability of the complexes, conditions for mixing RNA and protein components before crystallization, effect of Se-Met on RNA/protein complex crystal quality. In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes.

Original languageEnglish (US)
Pages (from-to)1664-1669
Number of pages6
JournalActa Crystallographica Section D: Biological Crystallography
Volume58
Issue number10 II
DOIs
Publication statusPublished - Oct 1 2002

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All Science Journal Classification (ASJC) codes

  • Structural Biology

Cite this

Garber, M., Gongadze, G., Meshcheryakov, V., Nikonov, O., Nikulin, A., Perederina, A., ... Tishchenko, S. (2002). Crystallization of RNA/protein complexes. Acta Crystallographica Section D: Biological Crystallography, 58(10 II), 1664-1669. https://doi.org/10.1107/S0907444902014257