Different complexes of ribosomal proteins with specific rRNA fragments have been crystallized and studied by our group during the last six years. There are several factors important for successful crystallization of RNA/protein complexes, among them: length and content of RNA fragments, homogeneity of RNA and protein preparations, stability of the complexes, conditions for mixing RNA and protein components before crystallization, effect of Se-Met on RNA/protein complex crystal quality. In this paper we describe findings and methodical details, which helped us to succeed in obtaining X-ray quality crystals of several RNA/protein complexes.
|Original language||English (US)|
|Number of pages||6|
|Journal||Acta Crystallographica Section D: Biological Crystallography|
|Issue number||10 II|
|Publication status||Published - Oct 1 2002|
All Science Journal Classification (ASJC) codes
- Structural Biology