Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA

Erica L. Schwalm, Tyler L. Grove, Squire J. Booker, Amie K. Boal

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

RlmN is a dual-specificity RNA methylase that modifies C2 of adenosine 2503 (A2503) in 23S rRNA and C2 of adenosine 37 (A37) in several Escherichia coli transfer RNAs (tRNAs). A related methylase, Cfr, modifies C8 of A2503 via a similar mechanism, conferring resistance to multiple classes of antibiotics. Here, we report the x-ray structure of a key intermediate in the RlmN reaction, in which a Cys118→Ala variant of the protein is cross-linked to a tRNAGlu substrate through the terminal methylene carbon of a formerly methylcysteinyl residue and C2 of A37. RlmN contacts the entire length of tRNAGlu, accessing A37 by using an induced-fit strategy that completely unfolds the tRNA anticodon stem-loop, which is likely critical for recognition of both tRNA and ribosomal RNA substrates.

Original languageEnglish (US)
Pages (from-to)309-312
Number of pages4
JournalScience
Volume352
Issue number6283
DOIs
StatePublished - Apr 15 2016

All Science Journal Classification (ASJC) codes

  • General

Fingerprint Dive into the research topics of 'Crystallographic capture of a radical S-adenosylmethionine enzyme in the act of modifying tRNA'. Together they form a unique fingerprint.

Cite this