Culture and regeneration of mesophyll-derived protoplasts of sorghum [Sorghum bicolor (L.) Moench]

R. V. Sairam, N. Seetharama, P. S. Devi, A. Verma, U. R. Murthy, I. Potrykus

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

A protocol for plant regeneration from mesophyll/protoplasts of sorghum [Sorghum bicolor (L.) Moench] was developed. The yield of intact protoplasts, their subsequent divisions and regeneration were genotype-dependent. The genotype 296B was always more responsive than IS 32266. For 296B, the sixth leaf from 18-day-old plants kept in dark for 2 days before harvesting was found to be the most suitable source of viable protoplasts. The first division was observed 10-12 days after plating, and the second division after 12-14 days. The maximum plating efficiency was 4.8% in 296 B, followed by 2.48% in IS 32266. Microcolonies were visible after 25-30 days, and microcalli after 60-75 days. Whole plants were obtained after 6-8 weeks of culture of microcalli on MS medium containing 0.2 mg l-1 kinetin and 2 mg l-1 BAP. The frequency of regeneration in 296B and IS 32266 was 12.80% and 10.58%, respectively. Ten plants transferred to pots in the glasshouse established well. The seeds collected from glasshouse-grown plants were sown in the field where plants were grown to maturity.

Original languageEnglish (US)
Pages (from-to)972-977
Number of pages6
JournalPlant Cell Reports
Volume18
Issue number12
DOIs
StatePublished - Sep 1 1999

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All Science Journal Classification (ASJC) codes

  • Agronomy and Crop Science
  • Plant Science

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