Cutting back malaria: CRISPR/Cas9 genome editing of Plasmodium

Marcus C.S. Lee, Scott E. Lindner, Jose Juan Lopez-Rubio, Manuel Llinás

Research output: Contribution to journalArticle

Abstract

CRISPR/Cas9 approaches are revolutionizing our ability to perform functional genomics across a wide range of organisms, including the Plasmodium parasites that cause malaria. The ability to deliver single point mutations, epitope tags and gene deletions at increased speed and scale is enabling our understanding of the biology of these complex parasites, and pointing to potential new therapeutic targets. In this review, we describe some of the biological and technical considerations for designing CRISPR-based experiments, and discuss potential future developments that broaden the applications for CRISPR/Cas9 interrogation of the malaria parasite genome.

Original languageEnglish (US)
Pages (from-to)281-289
Number of pages9
JournalBriefings in functional genomics
Volume18
Issue number5
DOIs
StatePublished - Sep 24 2019

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Clustered Regularly Interspaced Short Palindromic Repeats
Plasmodium
Malaria
Parasites
Genes
Gene Deletion
Genomics
Point Mutation
Epitopes
Genome
Gene Editing
Experiments
Therapeutics

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

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Cutting back malaria : CRISPR/Cas9 genome editing of Plasmodium. / Lee, Marcus C.S.; Lindner, Scott E.; Lopez-Rubio, Jose Juan; Llinás, Manuel.

In: Briefings in functional genomics, Vol. 18, No. 5, 24.09.2019, p. 281-289.

Research output: Contribution to journalArticle

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