Cutting edge: Drebrin-regulated actin dynamics regulate ige-dependent mast cell activation and allergic responses

Mankit Law, Yong Chan Lee, J. Luis Morales, Gang Ning, Weishan Huang, Jonathan Pabon, Arun K. Kannan, Ah Reum Jeong, Amie Wood, Chavez Carter, Sonia Mohinta, Jihong Song, Avery August

Research output: Contribution to journalReview article

8 Scopus citations

Abstract

Mast cells play critical roles in allergic responses. Calcium signaling controls the function of these cells, and a role for actin in regulating calcium influx into cells has been suggested. We have previously identified the actin reorganizing protein Drebrin as a target of the immunosuppressant 3,5-bistrifluoromethyl pyrazole, which inhibits calcium influx into cells. In this study, we show that Drebrin-/-2 mice exhibit reduced IgE-mediated histamine release and passive systemic anaphylaxis, and Drebrin-/-2 mast cells also exhibit defects in FceRI-mediated degranulation. Drebrin-/-2 mast cells exhibit defects in actin cytoskeleton organization and calcium responses downstream of the FceRI, and agents that relieve actin reorganization rescue mast cell FceRI-induced degranulation. Our results indicate that Drebrin regulates the actin cytoskeleton and calcium responses in mast cells, thus regulating mast cell function in vivo.

Original languageEnglish (US)
Pages (from-to)426-430
Number of pages5
JournalJournal of Immunology
Volume195
Issue number2
DOIs
StatePublished - Jul 15 2015

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology

Cite this

Law, M., Lee, Y. C., Luis Morales, J., Ning, G., Huang, W., Pabon, J., Kannan, A. K., Jeong, A. R., Wood, A., Carter, C., Mohinta, S., Song, J., & August, A. (2015). Cutting edge: Drebrin-regulated actin dynamics regulate ige-dependent mast cell activation and allergic responses. Journal of Immunology, 195(2), 426-430. https://doi.org/10.4049/jimmunol.1401442