Abstract

Chi, 5′ G-C-T-G-G-T-G-G 3′, stimulates coliphage λ recombination mediated by the major recombination pathway of Escherichia coli, the RecBC pathway. Purified RecBCD enzyme makes a single strand endonuclease cleavage four to six nucleotides to the 3′ side of the chi sequence. Three sequences similar to chi, 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, have partial recombinational hotspot activity in genetic crosses. We report here that purified RecBCD enzyme preferentially cuts four nucleotides to the right of the two most active chi-like octamers. The degree of cutting correlated with the genetic activities of these sequences; this result indicates that these cleavages are essential to the genetic activity of chi and chi-like sequences.

Original languageEnglish (US)
Pages (from-to)747-750
Number of pages4
JournalJournal of Molecular Biology
Volume194
Issue number4
DOIs
StatePublished - Apr 20 1987

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Exodeoxyribonuclease V
Genetic Recombination
Nucleotides
Genetic Crosses
Coliphages
Escherichia coli
Endonucleases

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

@article{eae64769efae4fc6952a2f9316e8b90a,
title = "Cutting of chi-like sequences by the RecBCD enzyme of Escherichia coli",
abstract = "Chi, 5′ G-C-T-G-G-T-G-G 3′, stimulates coliphage λ recombination mediated by the major recombination pathway of Escherichia coli, the RecBC pathway. Purified RecBCD enzyme makes a single strand endonuclease cleavage four to six nucleotides to the 3′ side of the chi sequence. Three sequences similar to chi, 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, have partial recombinational hotspot activity in genetic crosses. We report here that purified RecBCD enzyme preferentially cuts four nucleotides to the right of the two most active chi-like octamers. The degree of cutting correlated with the genetic activities of these sequences; this result indicates that these cleavages are essential to the genetic activity of chi and chi-like sequences.",
author = "Keith Cheng and Smith, {Gerald R.}",
year = "1987",
month = "4",
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language = "English (US)",
volume = "194",
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journal = "Journal of Molecular Biology",
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}

Cutting of chi-like sequences by the RecBCD enzyme of Escherichia coli. / Cheng, Keith; Smith, Gerald R.

In: Journal of Molecular Biology, Vol. 194, No. 4, 20.04.1987, p. 747-750.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cutting of chi-like sequences by the RecBCD enzyme of Escherichia coli

AU - Cheng, Keith

AU - Smith, Gerald R.

PY - 1987/4/20

Y1 - 1987/4/20

N2 - Chi, 5′ G-C-T-G-G-T-G-G 3′, stimulates coliphage λ recombination mediated by the major recombination pathway of Escherichia coli, the RecBC pathway. Purified RecBCD enzyme makes a single strand endonuclease cleavage four to six nucleotides to the 3′ side of the chi sequence. Three sequences similar to chi, 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, have partial recombinational hotspot activity in genetic crosses. We report here that purified RecBCD enzyme preferentially cuts four nucleotides to the right of the two most active chi-like octamers. The degree of cutting correlated with the genetic activities of these sequences; this result indicates that these cleavages are essential to the genetic activity of chi and chi-like sequences.

AB - Chi, 5′ G-C-T-G-G-T-G-G 3′, stimulates coliphage λ recombination mediated by the major recombination pathway of Escherichia coli, the RecBC pathway. Purified RecBCD enzyme makes a single strand endonuclease cleavage four to six nucleotides to the 3′ side of the chi sequence. Three sequences similar to chi, 5′ A-C-T-G-G-T-G-G 3′, 5′ G-T-T-G-G-T-G-G 3′ and 5′ G-C-T-A-G-T-G-G 3′, have partial recombinational hotspot activity in genetic crosses. We report here that purified RecBCD enzyme preferentially cuts four nucleotides to the right of the two most active chi-like octamers. The degree of cutting correlated with the genetic activities of these sequences; this result indicates that these cleavages are essential to the genetic activity of chi and chi-like sequences.

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