TY - JOUR
T1 - Cysteine residues reduce the severity of dopamine electrochemical fouling
AU - Harreither, Wolfgang
AU - Trouillon, Raphaël
AU - Poulin, Philippe
AU - Neri, Wilfrid
AU - Ewing, Andrew G.
AU - Safina, Gulnara
N1 - Funding Information:
The work has been supported by the European Research Council (Advanced Grant), the Knut and Alice Wallenberg Foundation, the Swedish Research Council (VR), The Royal Society of Arts and Sciences in Gothenburg and the National Institutes of Health. GS has been supported by a Young Investigator Project Grant from VR (621-2011-4395).
Publisher Copyright:
© 2016 Elsevier Ltd. All rights reserved.
PY - 2016/8/20
Y1 - 2016/8/20
N2 - The fouling of neurotransmitter during electrochemical detection can dramatically jeopardize the viability of the sensor. Several recent developments in electrode fabrication and design have helped mitigating this issue. For instance, carbon nanotube fiber (CNTF) electrodes were found to be more resistant to fouling than the traditional carbon fiber (CF) electrodes. Here, we investigate the fouling resistance of these two materials in the presence of albumin. Interestingly, our data shows that the presence of albumin reduces the impact of dopamine (DA) fouling in a dose-dependent manner. A protective effect from DA fouling was also observed for other thiol containing substances, thus hinting that the sulfur competes with the amine moiety for the nucleophilic binding to the oxidized catechol, a critical step initiating DA fouling, and therefore reduces the rate of DA polymerization on the electrode surface. Overall, this study furthers our understanding of the mechanisms controlling DA detection in situ, and suggests using solutions of albumin as a background for calibrating sensors.
AB - The fouling of neurotransmitter during electrochemical detection can dramatically jeopardize the viability of the sensor. Several recent developments in electrode fabrication and design have helped mitigating this issue. For instance, carbon nanotube fiber (CNTF) electrodes were found to be more resistant to fouling than the traditional carbon fiber (CF) electrodes. Here, we investigate the fouling resistance of these two materials in the presence of albumin. Interestingly, our data shows that the presence of albumin reduces the impact of dopamine (DA) fouling in a dose-dependent manner. A protective effect from DA fouling was also observed for other thiol containing substances, thus hinting that the sulfur competes with the amine moiety for the nucleophilic binding to the oxidized catechol, a critical step initiating DA fouling, and therefore reduces the rate of DA polymerization on the electrode surface. Overall, this study furthers our understanding of the mechanisms controlling DA detection in situ, and suggests using solutions of albumin as a background for calibrating sensors.
UR - http://www.scopus.com/inward/record.url?scp=84973596629&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84973596629&partnerID=8YFLogxK
U2 - 10.1016/j.electacta.2016.05.124
DO - 10.1016/j.electacta.2016.05.124
M3 - Article
AN - SCOPUS:84973596629
VL - 210
SP - 622
EP - 629
JO - Electrochimica Acta
JF - Electrochimica Acta
SN - 0013-4686
ER -