Cystometric changes in alloxan diabetic rats: evidence for functional and structural correlates of diabetic autonomic neuropathy

Maurizio Paro, Gianpaolo Italiano, Renato A. Travagli, Lucia Petrelli, Renzo Zanoni, Marco Prosdocimi, Mario G. Fiori

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Abstract

Autonomic neuropathy and urinary bladder function were compared in Sprague-Dawley rats with alloxan-diabetes of 3 months duration, rats fed sucrose for 8 weeks, and rats examined 8 weeks after pelvic nerve surgical axotomy; normal age-matched rats were used as controls. All experimental interventions induced bladder hypertrophy with increased bladder weight. In diabetic and sucrose-fed animals, water intake and urinary output increased. Cystometric recordings of normal rats in vivo showed rhythmic contractions (1.25 ± 0.25 contr/min) with threshold volume for micturition reflex at 0.51 ± 0.04 ml. In diabetic rats, bladder contractions were irregular and of lower frequency (0.60 ± 0.04 contr/min), while threshold volume was significantly higher (1.00 ± 0.11 ml). Bladder contractions were normal in sucrose-fed animals, though threshold volume was markedly augmented (1.27 ± 0.19 ml). Pelvic nerve surgical ablation abolished micturition reflex. In bladder strips excised post-mortem, contractile response to field stimulation was reduced in diabetic rats compared to control and sucrose-fed animals. Morphological examination of pelvic and hypogastric nerves revealed abnormalities characteristic of diabetic neuropathy only in diabetic rats. These data suggest that in alloxan-induced diabetes the decrease in the rate of bladder contraction is the result of autonomic neuropathy; while bladder hypertrophy in sucrose-fed rats appears to be an organ adaptation to hyperdiuresis.

Original languageEnglish (US)
Pages (from-to)1-11
Number of pages11
JournalJournal of the Autonomic Nervous System
Volume30
Issue number1
DOIs
StatePublished - Apr 1990

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Alloxan
Diabetic Neuropathies
Urinary Bladder
Sucrose
Experimental Diabetes Mellitus
Urination
Hypertrophy
Reflex
Axotomy
Gynecological Examination
Drinking
Sprague Dawley Rats
Weights and Measures

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Physiology
  • Clinical Neurology

Cite this

Paro, Maurizio ; Italiano, Gianpaolo ; Travagli, Renato A. ; Petrelli, Lucia ; Zanoni, Renzo ; Prosdocimi, Marco ; Fiori, Mario G. / Cystometric changes in alloxan diabetic rats : evidence for functional and structural correlates of diabetic autonomic neuropathy. In: Journal of the Autonomic Nervous System. 1990 ; Vol. 30, No. 1. pp. 1-11.
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abstract = "Autonomic neuropathy and urinary bladder function were compared in Sprague-Dawley rats with alloxan-diabetes of 3 months duration, rats fed sucrose for 8 weeks, and rats examined 8 weeks after pelvic nerve surgical axotomy; normal age-matched rats were used as controls. All experimental interventions induced bladder hypertrophy with increased bladder weight. In diabetic and sucrose-fed animals, water intake and urinary output increased. Cystometric recordings of normal rats in vivo showed rhythmic contractions (1.25 ± 0.25 contr/min) with threshold volume for micturition reflex at 0.51 ± 0.04 ml. In diabetic rats, bladder contractions were irregular and of lower frequency (0.60 ± 0.04 contr/min), while threshold volume was significantly higher (1.00 ± 0.11 ml). Bladder contractions were normal in sucrose-fed animals, though threshold volume was markedly augmented (1.27 ± 0.19 ml). Pelvic nerve surgical ablation abolished micturition reflex. In bladder strips excised post-mortem, contractile response to field stimulation was reduced in diabetic rats compared to control and sucrose-fed animals. Morphological examination of pelvic and hypogastric nerves revealed abnormalities characteristic of diabetic neuropathy only in diabetic rats. These data suggest that in alloxan-induced diabetes the decrease in the rate of bladder contraction is the result of autonomic neuropathy; while bladder hypertrophy in sucrose-fed rats appears to be an organ adaptation to hyperdiuresis.",
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Cystometric changes in alloxan diabetic rats : evidence for functional and structural correlates of diabetic autonomic neuropathy. / Paro, Maurizio; Italiano, Gianpaolo; Travagli, Renato A.; Petrelli, Lucia; Zanoni, Renzo; Prosdocimi, Marco; Fiori, Mario G.

In: Journal of the Autonomic Nervous System, Vol. 30, No. 1, 04.1990, p. 1-11.

Research output: Contribution to journalArticle

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T1 - Cystometric changes in alloxan diabetic rats

T2 - evidence for functional and structural correlates of diabetic autonomic neuropathy

AU - Paro, Maurizio

AU - Italiano, Gianpaolo

AU - Travagli, Renato A.

AU - Petrelli, Lucia

AU - Zanoni, Renzo

AU - Prosdocimi, Marco

AU - Fiori, Mario G.

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N2 - Autonomic neuropathy and urinary bladder function were compared in Sprague-Dawley rats with alloxan-diabetes of 3 months duration, rats fed sucrose for 8 weeks, and rats examined 8 weeks after pelvic nerve surgical axotomy; normal age-matched rats were used as controls. All experimental interventions induced bladder hypertrophy with increased bladder weight. In diabetic and sucrose-fed animals, water intake and urinary output increased. Cystometric recordings of normal rats in vivo showed rhythmic contractions (1.25 ± 0.25 contr/min) with threshold volume for micturition reflex at 0.51 ± 0.04 ml. In diabetic rats, bladder contractions were irregular and of lower frequency (0.60 ± 0.04 contr/min), while threshold volume was significantly higher (1.00 ± 0.11 ml). Bladder contractions were normal in sucrose-fed animals, though threshold volume was markedly augmented (1.27 ± 0.19 ml). Pelvic nerve surgical ablation abolished micturition reflex. In bladder strips excised post-mortem, contractile response to field stimulation was reduced in diabetic rats compared to control and sucrose-fed animals. Morphological examination of pelvic and hypogastric nerves revealed abnormalities characteristic of diabetic neuropathy only in diabetic rats. These data suggest that in alloxan-induced diabetes the decrease in the rate of bladder contraction is the result of autonomic neuropathy; while bladder hypertrophy in sucrose-fed rats appears to be an organ adaptation to hyperdiuresis.

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