Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2

Sébastien Alphonse, Jamie Jon Arnold, Shibani Bhattacharya, Hsin Wang, Brian Kloss, Craig Eugene Cameron, Ranajeet Ghose

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

In bacteriophages of the cystovirus family, the polymerase complex (PX) encodes a 75-kDa RNA-directed RNA polymerase (P2) that transcribes the double-stranded RNA genome. Also a constituent of the PX is the essential protein P7 that, in addition to accelerating PX assembly and facilitating genome packaging, plays a regulatory role in transcription. Deletion of P7 from the PX leads to aberrant plus-strand synthesis suggesting its influence on the transcriptase activity of P2. Here, using solution NMR techniques and the P2 and P7 proteins from cystovirus ψ12, we demonstrate their largely electrostatic interaction in vitro. Chemical shift perturbations on P7 in the presence of P2 suggest that this interaction involves the dynamic C-terminal tail of P7, more specifically an acidic cluster therein. Patterns of chemical shift changes induced on P2 by the P7 C-terminus resemble those seen in the presence of single-stranded RNA suggesting similarities in binding. This association between P2 and P7 reduces the affinity of the former toward template RNA and results in its decreased activity both in de novo RNA synthesis and in extending a short primer. Given the presence of C-terminal acidic tracts on all cystoviral P7 proteins, the electrostatic nature of the P2/P7 interaction is likely conserved within the family and could constitute a mechanism through which P7 regulates transcription in cystoviruses.

Original languageEnglish (US)
Pages (from-to)2580-2593
Number of pages14
JournalJournal of Molecular Biology
Volume426
Issue number14
DOIs
StatePublished - Jul 15 2014

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Cystoviridae
RNA Replicase
RNA
Static Electricity
Genome
Proteins
Double-Stranded RNA
Product Packaging
DNA-Directed RNA Polymerases
Bacteriophages

All Science Journal Classification (ASJC) codes

  • Molecular Biology

Cite this

Alphonse, S., Arnold, J. J., Bhattacharya, S., Wang, H., Kloss, B., Cameron, C. E., & Ghose, R. (2014). Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2. Journal of Molecular Biology, 426(14), 2580-2593. https://doi.org/10.1016/j.jmb.2014.04.028
Alphonse, Sébastien ; Arnold, Jamie Jon ; Bhattacharya, Shibani ; Wang, Hsin ; Kloss, Brian ; Cameron, Craig Eugene ; Ghose, Ranajeet. / Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2. In: Journal of Molecular Biology. 2014 ; Vol. 426, No. 14. pp. 2580-2593.
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Alphonse, S, Arnold, JJ, Bhattacharya, S, Wang, H, Kloss, B, Cameron, CE & Ghose, R 2014, 'Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2', Journal of Molecular Biology, vol. 426, no. 14, pp. 2580-2593. https://doi.org/10.1016/j.jmb.2014.04.028

Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2. / Alphonse, Sébastien; Arnold, Jamie Jon; Bhattacharya, Shibani; Wang, Hsin; Kloss, Brian; Cameron, Craig Eugene; Ghose, Ranajeet.

In: Journal of Molecular Biology, Vol. 426, No. 14, 15.07.2014, p. 2580-2593.

Research output: Contribution to journalArticle

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T1 - Cystoviral polymerase complex protein P7 uses its acidic C-terminal tail to regulate the RNA-directed RNA polymerase P2

AU - Alphonse, Sébastien

AU - Arnold, Jamie Jon

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AU - Kloss, Brian

AU - Cameron, Craig Eugene

AU - Ghose, Ranajeet

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