Cytochemical assay to localize respiratory activity in hairy root cultures

Usha Mukundan, Patrick T. Asplund, Wayne R. Curtis

Research output: Contribution to specialist publicationArticle

Abstract

When treated with nitroblue tetrazolium the hairy root cultures of Beta vulgaris (golden and red varieties), Brassica juncea, Cosmos sulphureus, Hyoscyamus muticus, Ocimum basilicum and Nicotiana tabaccum in Gamborg's liquid medium showed rapid and intense staining at the apical meristem and lateral root initials; this is indicative of elevated respiratory activity in these regions. The root hairs did not show any staining indicating their limited role in the overall respiratory budget of hairy roots grown as submerged cultures.

Original languageEnglish (US)
Pages184-187
Number of pages4
Volume50
No.2
Specialist publicationPhytomorphology: An International Journal of Plant Morphology
Publication statusPublished - Jan 1 2000

    Fingerprint

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this