Cytochemical assay to localize respiratory activity in hairy root cultures

Usha Mukundan, Patrick T. Asplund, Wayne R. Curtis

Research output: Contribution to specialist publicationArticle

Abstract

When treated with nitroblue tetrazolium the hairy root cultures of Beta vulgaris (golden and red varieties), Brassica juncea, Cosmos sulphureus, Hyoscyamus muticus, Ocimum basilicum and Nicotiana tabaccum in Gamborg's liquid medium showed rapid and intense staining at the apical meristem and lateral root initials; this is indicative of elevated respiratory activity in these regions. The root hairs did not show any staining indicating their limited role in the overall respiratory budget of hairy roots grown as submerged cultures.

Original languageEnglish (US)
Pages184-187
Number of pages4
Volume50
No2
Specialist publicationPhytomorphology: An International Journal of Plant Morphology
StatePublished - Jan 1 2000

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Cosmos sulphureus
assays
Hyoscyamus muticus
nitroblue tetrazolium
Ocimum basilicum
Beta vulgaris
root hairs
Brassica juncea
apical meristems
Nicotiana tabacum
liquids
staining

All Science Journal Classification (ASJC) codes

  • Plant Science

Cite this

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Cytochemical assay to localize respiratory activity in hairy root cultures. / Mukundan, Usha; Asplund, Patrick T.; Curtis, Wayne R.

In: Phytomorphology: An International Journal of Plant Morphology, Vol. 50, No. 2, 01.01.2000, p. 184-187.

Research output: Contribution to specialist publicationArticle

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