Cytochrome P-450 Optical Difference Spectra of Insecticides. A Comparative Study

Aran P. Kulkarni, Richard Mailman, Ernest Hodgson

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The cytochrome P-450 optical difference spectra of insecticides were examined using hepatic microsomes from sheep, rabbit, rat, and mouse as well as abdominal microsomes from insecticideresistant (Fc) and insecticide-susceptible (CSMA) house flies. The results generally conform to the hypothesis that type II binding spectra are elicited by compounds containing a nitrogen atom with a sterically accessible pair of nonbonded electrons and, in addition, certain compounds with nucleophilic oxygen atoms. Other compounds generally exhibit type I spectral binding or do not form detectable spectra. Exceptions include certain pyrethroids which, while giving typical type I difference spectra with mammalian microsomes, gave rise to an unusual spectrum in insects, one with a peak at 415-418 nm and trough at 445-447 nm. Rotenone also gave rise to unusual difference spectra with hepatic microsomes of sheep and rabbit. Differences in the ratios of spectral size between species indicate that qualitative differences in the cytochrome P-450 of different species are common.

Original languageEnglish (US)
Pages (from-to)177-183
Number of pages7
JournalJournal of agricultural and food chemistry
Volume23
Issue number2
DOIs
StatePublished - Mar 1 1975

Fingerprint

liver microsomes
microsomes
Insecticides
Microsomes
cytochrome P-450
Cytochrome P-450 Enzyme System
insecticides
rabbits
sheep
Rotenone
rotenone
Atoms
Pyrethrins
Carrier sense multiple access
Musca domestica
pyrethrins
Rats
Sheep
Nitrogen
electrons

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Agricultural and Biological Sciences(all)

Cite this

@article{cb35b564344d432c8735a9d1018964db,
title = "Cytochrome P-450 Optical Difference Spectra of Insecticides. A Comparative Study",
abstract = "The cytochrome P-450 optical difference spectra of insecticides were examined using hepatic microsomes from sheep, rabbit, rat, and mouse as well as abdominal microsomes from insecticideresistant (Fc) and insecticide-susceptible (CSMA) house flies. The results generally conform to the hypothesis that type II binding spectra are elicited by compounds containing a nitrogen atom with a sterically accessible pair of nonbonded electrons and, in addition, certain compounds with nucleophilic oxygen atoms. Other compounds generally exhibit type I spectral binding or do not form detectable spectra. Exceptions include certain pyrethroids which, while giving typical type I difference spectra with mammalian microsomes, gave rise to an unusual spectrum in insects, one with a peak at 415-418 nm and trough at 445-447 nm. Rotenone also gave rise to unusual difference spectra with hepatic microsomes of sheep and rabbit. Differences in the ratios of spectral size between species indicate that qualitative differences in the cytochrome P-450 of different species are common.",
author = "Kulkarni, {Aran P.} and Richard Mailman and Ernest Hodgson",
year = "1975",
month = "3",
day = "1",
doi = "10.1021/jf60198a054",
language = "English (US)",
volume = "23",
pages = "177--183",
journal = "Journal of Agricultural and Food Chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",
number = "2",

}

Cytochrome P-450 Optical Difference Spectra of Insecticides. A Comparative Study. / Kulkarni, Aran P.; Mailman, Richard; Hodgson, Ernest.

In: Journal of agricultural and food chemistry, Vol. 23, No. 2, 01.03.1975, p. 177-183.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cytochrome P-450 Optical Difference Spectra of Insecticides. A Comparative Study

AU - Kulkarni, Aran P.

AU - Mailman, Richard

AU - Hodgson, Ernest

PY - 1975/3/1

Y1 - 1975/3/1

N2 - The cytochrome P-450 optical difference spectra of insecticides were examined using hepatic microsomes from sheep, rabbit, rat, and mouse as well as abdominal microsomes from insecticideresistant (Fc) and insecticide-susceptible (CSMA) house flies. The results generally conform to the hypothesis that type II binding spectra are elicited by compounds containing a nitrogen atom with a sterically accessible pair of nonbonded electrons and, in addition, certain compounds with nucleophilic oxygen atoms. Other compounds generally exhibit type I spectral binding or do not form detectable spectra. Exceptions include certain pyrethroids which, while giving typical type I difference spectra with mammalian microsomes, gave rise to an unusual spectrum in insects, one with a peak at 415-418 nm and trough at 445-447 nm. Rotenone also gave rise to unusual difference spectra with hepatic microsomes of sheep and rabbit. Differences in the ratios of spectral size between species indicate that qualitative differences in the cytochrome P-450 of different species are common.

AB - The cytochrome P-450 optical difference spectra of insecticides were examined using hepatic microsomes from sheep, rabbit, rat, and mouse as well as abdominal microsomes from insecticideresistant (Fc) and insecticide-susceptible (CSMA) house flies. The results generally conform to the hypothesis that type II binding spectra are elicited by compounds containing a nitrogen atom with a sterically accessible pair of nonbonded electrons and, in addition, certain compounds with nucleophilic oxygen atoms. Other compounds generally exhibit type I spectral binding or do not form detectable spectra. Exceptions include certain pyrethroids which, while giving typical type I difference spectra with mammalian microsomes, gave rise to an unusual spectrum in insects, one with a peak at 415-418 nm and trough at 445-447 nm. Rotenone also gave rise to unusual difference spectra with hepatic microsomes of sheep and rabbit. Differences in the ratios of spectral size between species indicate that qualitative differences in the cytochrome P-450 of different species are common.

UR - http://www.scopus.com/inward/record.url?scp=0016636235&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016636235&partnerID=8YFLogxK

U2 - 10.1021/jf60198a054

DO - 10.1021/jf60198a054

M3 - Article

C2 - 1133289

AN - SCOPUS:0016636235

VL - 23

SP - 177

EP - 183

JO - Journal of Agricultural and Food Chemistry

JF - Journal of Agricultural and Food Chemistry

SN - 0021-8561

IS - 2

ER -