Cytogenetic engineering in vivo: Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells

N. L. Levy, R. Snyderman, R. L. Ladda, R. Lieberman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.

Original languageEnglish (US)
Pages (from-to)3125-3129
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume70
Issue number11
DOIs
StatePublished - Jan 1 1973

Fingerprint

Hybrid Cells
Cytogenetics
Macrophages
Feasibility Studies
Mammals
Chickens
Complement System Proteins
Erythrocytes
Kidney
Serum

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{b179d2ab89114d369075e20c0f00d96f,
title = "Cytogenetic engineering in vivo: Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells",
abstract = "Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.",
author = "Levy, {N. L.} and R. Snyderman and Ladda, {R. L.} and R. Lieberman",
year = "1973",
month = "1",
day = "1",
doi = "10.1073/pnas.70.11.3125",
language = "English (US)",
volume = "70",
pages = "3125--3129",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "11",

}

Cytogenetic engineering in vivo : Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells. / Levy, N. L.; Snyderman, R.; Ladda, R. L.; Lieberman, R.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 70, No. 11, 01.01.1973, p. 3125-3129.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Cytogenetic engineering in vivo

T2 - Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells

AU - Levy, N. L.

AU - Snyderman, R.

AU - Ladda, R. L.

AU - Lieberman, R.

PY - 1973/1/1

Y1 - 1973/1/1

N2 - Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.

AB - Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.

UR - http://www.scopus.com/inward/record.url?scp=0015743227&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0015743227&partnerID=8YFLogxK

U2 - 10.1073/pnas.70.11.3125

DO - 10.1073/pnas.70.11.3125

M3 - Article

C2 - 4131744

AN - SCOPUS:0015743227

VL - 70

SP - 3125

EP - 3129

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 11

ER -