Cytogenetic engineering in vivo: Restoration of biologic complement activity to C5 deficient mice by intravenous inoculation of hybrid cells

N. L. Levy, R. Snyderman, R. L. Ladda, R. Lieberman

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Splenic macrophages were identified as at least one source of C5 elaboration in normal mice. Hybrid cells were formed from splenic macrophages from C5 deficient mice and either kidney cells from mice with normal amounts of C5 or chicken erythrocytes. These hybrids elaborated C5 in vitro. C5 Deficient mice inoculated with these hybrid cells developed, in their serum, antigenically active mouse C5, as well as both hemolytic and biologic complement activity. These studies demonstrate the feasibility of genetic 'repair' in mammals.

Original languageEnglish (US)
Pages (from-to)3125-3129
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume70
Issue number11
DOIs
StatePublished - Jan 1 1973

All Science Journal Classification (ASJC) codes

  • General

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