Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family

Anton Nekrutenko, David M. Hillis, John C. Patton, Robert D. Bradley, Robert J. Baker

Research output: Contribution to journalArticle

59 Citations (Scopus)

Abstract

In this study, we report cDNA sequences of the cytosolic NADP-dependent isocitrate dehydrogenase for humans, mice, and two species of voles (Microtus mexicanus and Microtus ochrogaster). Inferred amino acid sequences from these taxa display a high level of amino acid sequence conservation, comparable to that of myosin β heavy chain, and share known structural features. A Caenorhabditis elegans enzyme that was previously identified as a protein similar to isocitrate dehydrogenase is most likely the NADP-dependent cytosolic isocitrate dehydrogenase enzyme equivalent, based on amino acid similarity to mammalian enzymes and phylogenetic analysis. We also suggest that NADP-dependent isocitrate dehydrogenases characterized from alfalfa, soybean, and eucalyptus are most likely cytosolic enzymes. The phylogenetic tree of various isocitrate dehydrogenases from eukaryotic sources revealed that independent gene duplications may have given rise to the cytosolic and mitochondrial forms of NADP-dependent isocitrate dehydrogenase in animals and fungi. There appears to be no statistical support for a hypothesis that the mitochondrial and cytosolic forms of the enzyme are orthologous in these groups. A possible scenario of the evolution of NADP-dependent isocitrate dehydrogenases is proposed.

Original languageEnglish (US)
Pages (from-to)1674-1684
Number of pages11
JournalMolecular biology and evolution
Volume15
Issue number12
DOIs
StatePublished - Dec 1998

Fingerprint

isocitrate dehydrogenase (NADP)
Isocitrate Dehydrogenase
isocitrate dehydrogenase
Arvicolinae
enzyme
phylogenetics
phylogeny
mice
Enzymes
enzymes
amino acid
Amino Acid Sequence
amino acid sequences
Microtus ochrogaster
Eucalyptus
Gene Duplication
Microtus
Medicago sativa
Myosin Heavy Chains
myosin heavy chains

All Science Journal Classification (ASJC) codes

  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics

Cite this

Nekrutenko, Anton ; Hillis, David M. ; Patton, John C. ; Bradley, Robert D. ; Baker, Robert J. / Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family. In: Molecular biology and evolution. 1998 ; Vol. 15, No. 12. pp. 1674-1684.
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Cytosolic isocitrate dehydrogenase in humans, mice, and voles and phylogenetic analysis of the enzyme family. / Nekrutenko, Anton; Hillis, David M.; Patton, John C.; Bradley, Robert D.; Baker, Robert J.

In: Molecular biology and evolution, Vol. 15, No. 12, 12.1998, p. 1674-1684.

Research output: Contribution to journalArticle

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