De novo emergence of a novel satellite RNA of cucumber mosaic virus following serial passages of the virus derived from RNA transcripts

M. R. Hajimorad, S. A. Ghabrial, M. J. Roossinck

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Satellite RNA (satRNA) is often associated with cucumber mosaic virus (CMV); however, its origin remains unexplained and a subject for speculation. We passaged progeny of molecularly cloned CMV-Fny and CMV-LS in Nicotiana tabacum cv. Ky 14 under greenhouse conditions. A satRNA emerged after at least eight successive transfers of CMV-Fny, but no satRNA was recovered after eleven serial transfers of CMV-LS under the same conditions. The sequences of the newly emerged satRNA were determined, and an infectious cDNA clone was synthesized. Comparison of the sequences of the newly emerged satRNA with those of known CMV satRNAs showed that it is unique. This observation raises interesting questions regarding the enigmatic nature of the origin of CMV satRNAs.

Original languageEnglish (US)
Pages (from-to)137-140
Number of pages4
JournalArchives of Virology
Volume154
Issue number1
DOIs
StatePublished - Jan 1 2009

Fingerprint

Cucumber Mosaic Virus Satellite
Cucumovirus
Serial Passage
Satellite RNAs
RNA Viruses
Tobacco
Complementary DNA
Clone Cells

All Science Journal Classification (ASJC) codes

  • Virology

Cite this

@article{001bf9db410d49448e0766d74c265f20,
title = "De novo emergence of a novel satellite RNA of cucumber mosaic virus following serial passages of the virus derived from RNA transcripts",
abstract = "Satellite RNA (satRNA) is often associated with cucumber mosaic virus (CMV); however, its origin remains unexplained and a subject for speculation. We passaged progeny of molecularly cloned CMV-Fny and CMV-LS in Nicotiana tabacum cv. Ky 14 under greenhouse conditions. A satRNA emerged after at least eight successive transfers of CMV-Fny, but no satRNA was recovered after eleven serial transfers of CMV-LS under the same conditions. The sequences of the newly emerged satRNA were determined, and an infectious cDNA clone was synthesized. Comparison of the sequences of the newly emerged satRNA with those of known CMV satRNAs showed that it is unique. This observation raises interesting questions regarding the enigmatic nature of the origin of CMV satRNAs.",
author = "Hajimorad, {M. R.} and Ghabrial, {S. A.} and Roossinck, {M. J.}",
year = "2009",
month = "1",
day = "1",
doi = "10.1007/s00705-008-0280-x",
language = "English (US)",
volume = "154",
pages = "137--140",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer Wien",
number = "1",

}

De novo emergence of a novel satellite RNA of cucumber mosaic virus following serial passages of the virus derived from RNA transcripts. / Hajimorad, M. R.; Ghabrial, S. A.; Roossinck, M. J.

In: Archives of Virology, Vol. 154, No. 1, 01.01.2009, p. 137-140.

Research output: Contribution to journalArticle

TY - JOUR

T1 - De novo emergence of a novel satellite RNA of cucumber mosaic virus following serial passages of the virus derived from RNA transcripts

AU - Hajimorad, M. R.

AU - Ghabrial, S. A.

AU - Roossinck, M. J.

PY - 2009/1/1

Y1 - 2009/1/1

N2 - Satellite RNA (satRNA) is often associated with cucumber mosaic virus (CMV); however, its origin remains unexplained and a subject for speculation. We passaged progeny of molecularly cloned CMV-Fny and CMV-LS in Nicotiana tabacum cv. Ky 14 under greenhouse conditions. A satRNA emerged after at least eight successive transfers of CMV-Fny, but no satRNA was recovered after eleven serial transfers of CMV-LS under the same conditions. The sequences of the newly emerged satRNA were determined, and an infectious cDNA clone was synthesized. Comparison of the sequences of the newly emerged satRNA with those of known CMV satRNAs showed that it is unique. This observation raises interesting questions regarding the enigmatic nature of the origin of CMV satRNAs.

AB - Satellite RNA (satRNA) is often associated with cucumber mosaic virus (CMV); however, its origin remains unexplained and a subject for speculation. We passaged progeny of molecularly cloned CMV-Fny and CMV-LS in Nicotiana tabacum cv. Ky 14 under greenhouse conditions. A satRNA emerged after at least eight successive transfers of CMV-Fny, but no satRNA was recovered after eleven serial transfers of CMV-LS under the same conditions. The sequences of the newly emerged satRNA were determined, and an infectious cDNA clone was synthesized. Comparison of the sequences of the newly emerged satRNA with those of known CMV satRNAs showed that it is unique. This observation raises interesting questions regarding the enigmatic nature of the origin of CMV satRNAs.

UR - http://www.scopus.com/inward/record.url?scp=58149305791&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=58149305791&partnerID=8YFLogxK

U2 - 10.1007/s00705-008-0280-x

DO - 10.1007/s00705-008-0280-x

M3 - Article

C2 - 19082686

AN - SCOPUS:58149305791

VL - 154

SP - 137

EP - 140

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 1

ER -