Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model

Omair A. Mohiuddin, Brett Campbell, J. Nick Poche, Michelle Ma, Emma Rogers, Dina Gaupp, Mark A.A. Harrison, Bruce A. Bunnell, Daniel J. Hayes, Jeffrey M. Gimble

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Critical-sized bone defects fail to heal and often cause non-union. Standard treatments employ autologous bone grafting, which can cause donor tissue loss/pain. Although several scaffold types can enhance bone regeneration, multiple factors limit their level of success. To address this issue, this study evaluated a novel decellularized human adipose tissue (DAT) hydrogel as an alternative. In this study, DAT hydrogel alone, or in combination with adipose-derived stromal/stem cells (ASC), osteo-induced ASCs (OIASC), and hydroxyapatite were tested for their ability to mediate repair of a critical-sized (3 mm) femoral defect created in C57BL/6 mice. Micro-computed tomography results showed that all DAT hydrogel treated groups significantly enhanced bone regeneration, with OIASC + hydroxyapatite treated group displaying the most robust bone regeneration. Histological analyses revealed that all treatments resulted in significantly higher tissue areas with the relative mineralized tissue area significantly increased at 12 weeks; however, cartilaginous content was lowest among treatment groups with OIASC. Immunohistochemical analyses showed that DAT hydrogel enhanced collagen I and osteopontin expression, while the addition of OIASCs to the hydrogel reduced collagen II levels. Thus, DAT hydrogel promotes bone regeneration in a critical-sized femoral defect model that is further enhanced in the presence of OIASCs and hydroxyapatite.

Original languageEnglish (US)
Article number211
JournalFrontiers in Bioengineering and Biotechnology
Volume7
DOIs
StatePublished - Sep 6 2019

Fingerprint

Bone Regeneration
Hydrogel
Thigh
Hydrogels
Adipose Tissue
Bone
Tissue
Defects
Durapatite
Hydroxyapatite
Collagen
Nociceptive Pain
Osteopontin
Bone Transplantation
Stromal Cells
Inbred C57BL Mouse
Stem Cells
Therapeutics
Stem cells
Tomography

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Bioengineering
  • Histology
  • Biomedical Engineering

Cite this

Mohiuddin, Omair A. ; Campbell, Brett ; Poche, J. Nick ; Ma, Michelle ; Rogers, Emma ; Gaupp, Dina ; Harrison, Mark A.A. ; Bunnell, Bruce A. ; Hayes, Daniel J. ; Gimble, Jeffrey M. / Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model. In: Frontiers in Bioengineering and Biotechnology. 2019 ; Vol. 7.
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Decellularized Adipose Tissue Hydrogel Promotes Bone Regeneration in Critical-Sized Mouse Femoral Defect Model. / Mohiuddin, Omair A.; Campbell, Brett; Poche, J. Nick; Ma, Michelle; Rogers, Emma; Gaupp, Dina; Harrison, Mark A.A.; Bunnell, Bruce A.; Hayes, Daniel J.; Gimble, Jeffrey M.

In: Frontiers in Bioengineering and Biotechnology, Vol. 7, 211, 06.09.2019.

Research output: Contribution to journalArticle

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AU - Mohiuddin, Omair A.

AU - Campbell, Brett

AU - Poche, J. Nick

AU - Ma, Michelle

AU - Rogers, Emma

AU - Gaupp, Dina

AU - Harrison, Mark A.A.

AU - Bunnell, Bruce A.

AU - Hayes, Daniel J.

AU - Gimble, Jeffrey M.

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