Defects in dendrite and spine maturation and synaptogenesis associated with an anxious-depressive-like phenotype of GABAA receptor-deficient mice

Zhen Ren, Nadia Sahir, Shoko Murakami, Beth A. Luellen, John C. Earnheart, Rachnanjali Lal, Ju Young Kim, Hongjun Song, Bernhard Luscher

Research output: Contribution to journalArticle

23 Scopus citations

Abstract

Mice that were rendered heterozygous for the γ2 subunit of GABAA receptors (γ2+/- mice) have been characterized extensively as a model for major depressive disorder. The phenotype of these mice includes behavior indicative of heightened anxiety, despair, and anhedonia, as well as defects in hippocampus-dependent pattern separation, HPA axis hyperactivity and increased responsiveness to antidepressant drugs. The γ2+/- model thereby provides strong support for the GABAergic deficit hypothesis of major depressive disorder. Here we show that γ2+/- mice additionally exhibit specific defects in late stage survival of adult-born hippocampal granule cells, including reduced complexity of dendritic arbors and impaired maturation of synaptic spines. Moreover, cortical γ2+/- neurons cultured in vitro show marked deficits in GABAergic innervation selectively when grown under competitive conditions that may mimic the environment of adult-born hippocampal granule cells. Finally, brain extracts of γ2+/- mice show a numerical but insignificant trend (p = 0.06) for transiently reduced expression of brain derived neurotrophic factor (BDNF) at three weeks of age, which might contribute to the previously reported developmental origin of the behavioral phenotype of γ2+/- mice. The data indicate increasing congruence of the GABAergic, glutamatergic, stress-based and neurotrophic deficit hypotheses of major depressive disorder. This article is part of the Special Issue entitled 'GABAergic Signaling in Health and Disease'.

Original languageEnglish (US)
Pages (from-to)171-179
Number of pages9
JournalNeuropharmacology
Volume88
DOIs
StatePublished - Jan 2015

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Cellular and Molecular Neuroscience

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