Degradation of pentachlorophenol by fixed films of white rot fungi in rotating tube bioreactors

Bruce C. Alleman, Bruce Ernest Logan, Robert L. Gilbertson

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

A rotating tube bioreactor (RTB) was developed to examine chemical degradation by fungal biofilms under controlled laboratory conditions. Each RTB consists of a sealed tube containing mycelia growing on a piece of steel mesh on the inside wall of the tube. Tubes are mounted horizontally in a tube rotator and slowly rotated to expose the fungal biofilm to a wetting-aeration cycle typical of fixed film reactors such as trickling filters and rotating biological contactors. The extent of pentachlorophenol (PCP) dehalogenation by three different species of fungal biofilms was evaluated by measuring concentrations of PCP (using HPLC) and the total concentrations of organic and inorganic halides (using a TOX analyzer). The three species of fungi removed PCP from solution at similar rates, but they differed in ability to completely dehalogenate PCP. Nitrogen-deficient growth media only slightly increased the extent of PCP mineralization. All species of fungi (grown on nitrogen-sufficient media) removed PCP to <0.1 mg l-1 (>99% removal) within 1 d. Within 8 d, the greatest percent of dehalogenation, measured by the appearance of chloride ion in solution, was achieved by Trametes versicolor (62%), followed by Phanerochaete chrysosporium (38%) and Inonotus dryophilus (21%). These removals based on chloride ion concentrations are comparable to claims by others of complete mineralization for recoveries of 10-50% of 14CO2 from 14C-labeled PCP in static flask cultures.

Original languageEnglish (US)
Pages (from-to)61-67
Number of pages7
JournalWater Research
Volume29
Issue number1
DOIs
StatePublished - Jan 1 1995

Fingerprint

PCP
Biofilms
Bioreactors
Fungi
Dehalogenation
bioreactor
fungus
Degradation
degradation
Nitrogen
biofilm
Ions
Wetting
Recovery
chloride
mineralization
Steel
ion
halide
nitrogen

All Science Journal Classification (ASJC) codes

  • Ecological Modeling
  • Water Science and Technology
  • Waste Management and Disposal
  • Pollution

Cite this

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abstract = "A rotating tube bioreactor (RTB) was developed to examine chemical degradation by fungal biofilms under controlled laboratory conditions. Each RTB consists of a sealed tube containing mycelia growing on a piece of steel mesh on the inside wall of the tube. Tubes are mounted horizontally in a tube rotator and slowly rotated to expose the fungal biofilm to a wetting-aeration cycle typical of fixed film reactors such as trickling filters and rotating biological contactors. The extent of pentachlorophenol (PCP) dehalogenation by three different species of fungal biofilms was evaluated by measuring concentrations of PCP (using HPLC) and the total concentrations of organic and inorganic halides (using a TOX analyzer). The three species of fungi removed PCP from solution at similar rates, but they differed in ability to completely dehalogenate PCP. Nitrogen-deficient growth media only slightly increased the extent of PCP mineralization. All species of fungi (grown on nitrogen-sufficient media) removed PCP to <0.1 mg l-1 (>99{\%} removal) within 1 d. Within 8 d, the greatest percent of dehalogenation, measured by the appearance of chloride ion in solution, was achieved by Trametes versicolor (62{\%}), followed by Phanerochaete chrysosporium (38{\%}) and Inonotus dryophilus (21{\%}). These removals based on chloride ion concentrations are comparable to claims by others of complete mineralization for recoveries of 10-50{\%} of 14CO2 from 14C-labeled PCP in static flask cultures.",
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Degradation of pentachlorophenol by fixed films of white rot fungi in rotating tube bioreactors. / Alleman, Bruce C.; Logan, Bruce Ernest; Gilbertson, Robert L.

In: Water Research, Vol. 29, No. 1, 01.01.1995, p. 61-67.

Research output: Contribution to journalArticle

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