@article{ddaa4703ef404b6d83fe1ab1ab1d2870,
title = "Dehydration prompts increased activity and blood feeding by mosquitoes",
abstract = "Current insights into the mosquito dehydration response rely on studies that examine specific responses but ultimately fail to provide an encompassing view of mosquito biology. Here, we examined underlying changes in the biology of mosquitoes associated with dehydration. Specifically, we show that dehydration increases blood feeding in the northern house mosquito, Culex pipiens, which was the result of both higher activity and a greater tendency to land on a host. Similar observations were noted for Aedes aegypti and Anopheles quadrimaculatus. RNA-seq and metabolome analyses in C. pipiens following dehydration revealed that factors associated with carbohydrate metabolism are altered, specifically the breakdown of trehalose. Suppression of trehalose breakdown in C. pipiens by RNA interference reduced phenotypes associated with lower hydration levels. Lastly, mesocosm studies for C. pipiens confirmed that dehydrated mosquitoes were more likely to host feed under ecologically relevant conditions. Disease modeling indicates dehydration bouts will likely enhance viral transmission. This dehydration-induced increase in blood feeding is therefore likely to occur regularly and intensify during periods when availability of water is low.",
author = "Hagan, {Richard W.} and Didion, {Elise M.} and Rosselot, {Andrew E.} and Holmes, {Christopher J.} and Siler, {Samantha C.} and Rosendale, {Andrew J.} and Hendershot, {Jacob M.} and Elliot, {Kiaira S.B.} and Jennings, {Emily C.} and Nine, {Gabriela A.} and Perez, {Paula L.} and Rizlallah, {Alexandre E.} and Miki Watanabe and Romick-Rosendale, {Lindsey E.} and Yanyu Xiao and Rasgon, {Jason L.} and Benoit, {Joshua B.}",
note = "Funding Information: Tis work was supported in part by a Faculty Development Grant from the University of Cincinnati (J.B.B.), Ohio Supercomputer Center (J.B.B.), United States Department of Agriculture's National Institute of Food and Agricultural Grant (2016-67012-24652, A.J.R.), partially supported by the NSF grant DEB-165441 (J.B.B.), NMRBased Metabolomics Core at the Cincinnati Children's Hospital Medical Center, and National Institutes of Health (R01AI116636 and R21AI128918, J.L.R.). Funding Information: We appreciate the helpful comments on early versions of the manuscript from David L. Denlinger (Ohio State University) and Brian L. Weiss (Yale University) and comments provided by the two anonymous reviewers. This work was supported in part by a Faculty Development Grant from the University of Cincinnati (J.B.B.), Ohio Supercomputer Center (J.B.B.), United States Department of Agriculture{\textquoteright}s National Institute of Food and Agricultural Grant (2016-67012-24652, A.J.R.), partially supported by the NSF grant DEB-165441 (J.B.B.), NMR-Based Metabolomics Core at the Cincinnati Children{\textquoteright}s Hospital Medical Center, and National Institutes of Health (R01AI116636 and R21AI128918, J.L.R.). Publisher Copyright: {\textcopyright} 2018 The Author(s).",
year = "2018",
month = dec,
day = "1",
doi = "10.1038/s41598-018-24893-z",
language = "English (US)",
volume = "8",
journal = "Scientific Reports",
issn = "2045-2322",
publisher = "Nature Publishing Group",
number = "1",
}