Delayed increase in neuronal nitric oxide synthase immunoreactivity in thalamus and other brain regions after hypoxic-ischemic injury in neonatal rats

Akira Ishida, Shun Ishiwa, William Trescher, Wako Nakajima, Mary S. Lange, Mary E. Blue, Michael V. Johnston

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

We examined the response of neuronal nitric oxide synthase (nNOS)-containing CNS neurons in rats exposed to a unilateral hypoxic-ischemic insult at 7 days of age. Animals were sacrificed at several time points after the injury, up to and including 7 days (Postnatal Day 14). Brain regions ipsilateral to the injury (including cerebral cortex, caudate-putamen, and thalamus) exhibited delayed, focal increases in nNOS immunoreactivity. The increase in nNOS immunoreactive fiber staining was prominent in areas adjacent to severe neuronal damage, especially in the cortex and the thalamus, regions that are also heavily and focally injured in term human neonates with hypoxic-ischemic encephalopathy. In cerebral cortex, these increases occurred despite modest declines in nNOS catalytic activity and protein levels. Proliferation of surviving nNOS immunoreactive fibers highlights regions of selective vulnerability to hypoxic-ischemic insult in the neonatal brain and may also contribute to plasticity of neuronal circuitry during recovery.

Original languageEnglish (US)
Pages (from-to)323-333
Number of pages11
JournalExperimental Neurology
Volume168
Issue number2
DOIs
StatePublished - Jan 1 2001

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Nitric Oxide Synthase Type I
Thalamus
Wounds and Injuries
Brain
Cerebral Cortex
Brain Hypoxia-Ischemia
Neuronal Plasticity
Putamen
Staining and Labeling
Neurons
Proteins

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience

Cite this

Ishida, Akira ; Ishiwa, Shun ; Trescher, William ; Nakajima, Wako ; Lange, Mary S. ; Blue, Mary E. ; Johnston, Michael V. / Delayed increase in neuronal nitric oxide synthase immunoreactivity in thalamus and other brain regions after hypoxic-ischemic injury in neonatal rats. In: Experimental Neurology. 2001 ; Vol. 168, No. 2. pp. 323-333.
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Delayed increase in neuronal nitric oxide synthase immunoreactivity in thalamus and other brain regions after hypoxic-ischemic injury in neonatal rats. / Ishida, Akira; Ishiwa, Shun; Trescher, William; Nakajima, Wako; Lange, Mary S.; Blue, Mary E.; Johnston, Michael V.

In: Experimental Neurology, Vol. 168, No. 2, 01.01.2001, p. 323-333.

Research output: Contribution to journalArticle

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