Delayed oligodendrocyte degeneration induced by brief exposure to hydrogen peroxide

Iwona Hirschler-Laszkiewicz, Raymond Mouzannar, Richard C. Wiggins, Gregory W. Konat

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

An in vitro model system of cultured oligodendrocytes was used to determine the susceptibility of these cells to oxidative stress induced by 15 min exposure to millimolar concentrations of hydrogen peroxide (H2O2). Following the exposure, the cells were incubated in normal growth medium, and analyzed at different time points. Although no cell loss was observed during the exposure period, there was a progressive depletion of adherent cells during the postexposure period as seen from either the number of recoverable nuclei, or from total RNA content of the cultures. Both the rate and the extent of cell deletion was directly dependent on H2O2 concentration. Cell death was preceded by structural alterations in the nuclear envelope resulting in 'fragile' nuclei which disintegrated during isolation. Northern blot analysis showed that the expression of myelin-specific genes was rapidly downregulated in H2O2-treated cells. On the other hand, the expression of antiapoptotic gene, bcl-2 featured massive but transient upregulation. Oligodendrocyte degeneration also featured genomic DNA degradation into high molecular weight fragments, which are likely to represent cleaved chromosomal loops. The results demonstrate vulnerability of oligodendrocytes to oxidative stress that induces rapid degeneration and ultimately leads to delayed cell death. This feature is highly relevant to oligodendrocyte damage and depletion following ischemic, traumatic, or inflammatory insults to the central nervous system (CNS).

Original languageEnglish (US)
Pages (from-to)303-310
Number of pages8
JournalJournal of Neuroscience Research
Volume55
Issue number3
DOIs
StatePublished - Feb 1 1999

All Science Journal Classification (ASJC) codes

  • Cellular and Molecular Neuroscience

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