Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia

Ashok K. Chockalingam, Danielle Hickman, Lindomar Pena, Jianqiang Ye, Andrea Ferrero, Jose R. Echenique, Hongjun Chen, Troy Sutton, Daniel R. Perez

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy.

Original languageEnglish (US)
Pages (from-to)3564-3573
Number of pages10
JournalJournal of virology
Volume86
Issue number7
DOIs
StatePublished - Apr 1 2012

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bacterial pneumonia
Bacterial Pneumonia
Influenza in Birds
sialidase
Neuraminidase
Orthomyxoviridae
Influenza A virus
Streptococcus pneumoniae
H9N2 Subtype Influenza A Virus
Viruses
mice
viruses
pneumonia
Pneumonia
lungs
Pneumococcal Pneumonia
Oseltamivir
vertebrate viruses
Pneumococcal Infections
adenoma

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Insect Science
  • Virology

Cite this

Chockalingam, Ashok K. ; Hickman, Danielle ; Pena, Lindomar ; Ye, Jianqiang ; Ferrero, Andrea ; Echenique, Jose R. ; Chen, Hongjun ; Sutton, Troy ; Perez, Daniel R. / Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia. In: Journal of virology. 2012 ; Vol. 86, No. 7. pp. 3564-3573.
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abstract = "We investigated the synergism between influenza virus and Streptococcus pneumoniae, particularly the role of deletions in the stalk region of the neuraminidase (NA) of H2N2 and H9N2 avian influenza viruses. Deletions in the NA stalk (ΔNA) had no effect on NA activity or on the adherence of S. pneumoniae to virus-infected human alveolar epithelial (A549) and mouse lung adenoma (LA-4) cells, although it delayed virus elution from turkey red blood cells. Sequential S. pneumoniae infection of mice previously inoculated with isogenic recombinant H2N2 and H9N2 influenza viruses displayed severe pneumonia, elevated levels of intrapulmonary proinflammatory responses, and death. No differences between the WT and ΔNA mutant viruses were detected with respect to effects on postinfluenza pneumococcal pneumonia as measured by bacterial growth, lung inflammation, morbidity, mortality, and cytokine/chemokine concentrations. Differences were observed, however, in influenza virus-infected mice that were treated with oseltamivir prior to a challenge with S. pneumoniae. Under these circumstances, mice infected with ΔNA viruses were associated with a better prognosis following a secondary bacterial challenge. These data suggest that the H2N2 and H9N2 subtypes of avian influenza A viruses can contribute to secondary bacterial pneumonia and deletions in the NA stalk may modulate its outcome in the context of antiviral therapy.",
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Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia. / Chockalingam, Ashok K.; Hickman, Danielle; Pena, Lindomar; Ye, Jianqiang; Ferrero, Andrea; Echenique, Jose R.; Chen, Hongjun; Sutton, Troy; Perez, Daniel R.

In: Journal of virology, Vol. 86, No. 7, 01.04.2012, p. 3564-3573.

Research output: Contribution to journalArticle

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T1 - Deletions in the neuraminidase stalk region of H2N2 and H9N2 avian influenza virus subtypes do not affect postinfluenza secondary bacterial pneumonia

AU - Chockalingam, Ashok K.

AU - Hickman, Danielle

AU - Pena, Lindomar

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AU - Sutton, Troy

AU - Perez, Daniel R.

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