Demethylation of ribosomal RNA by hepatocyte microsomal preparations

Won T. Choe, Christine L. Hatem, Gary A. Clawson

Research output: Contribution to journalArticlepeer-review

Abstract

Previous work has shown that hypomethylation of rRNA is an important control for protein synthesis in rat hepatocytes, and that the net hypomethylation appears to arise from cytoplasmic events. Here we show that demethylation of rRNA is catalyzed by microsomal preparations. The rRNA demethylation is dependent upon NADPH and is almost completely inhibited by carbon monoxide. Demethylation appears to increase following galactosamine intoxication, a hepatotoxin which induces hypomethylation of rRNA and inhibition of protein synthesis.

Original languageEnglish (US)
Pages (from-to)1585-1589
Number of pages5
JournalLife Sciences
Volume48
Issue number16
DOIs
StatePublished - 1991

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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