Demonstration by 2H ENDOR spectroscopy that myo-inositol binds via an alkoxide bridge to the mixed-valent diiron center of myo-inositol oxygenase

Sun Hee Kim, Gang Xing, J. Martin Bollinger, Carsten Krebs, Brian M. Hoffman

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

myo-Inositol oxygenase (MIOX) is a non-heme diiron oxygenase that cleaves cyclohexane-(1,2,3,5/4,6-hexa)-ol (myo-inositol, MI) to d-glucuronate. Here, we use 2H ENDOR spectroscopy to demonstrate that MI binds to the diiron(II/III) cofactor of MIOX via an alkoxide bridge, most likely involving O1. Analysis shows that MI adopts a symmetrical geometry in which the O-C-2H plane of the bridge is approximately orthogonal to the Fe-O-Fe plane.

Original languageEnglish (US)
Pages (from-to)10374-10375
Number of pages2
JournalJournal of the American Chemical Society
Volume128
Issue number32
DOIs
StatePublished - Aug 16 2006

All Science Journal Classification (ASJC) codes

  • Catalysis
  • Chemistry(all)
  • Biochemistry
  • Colloid and Surface Chemistry

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