TY - JOUR
T1 - Dendrite injury triggers DLK-independent regeneration
AU - Stone, Michelle C.
AU - Albertson, Richard M.
AU - Chen, Li
AU - Rolls, Melissa M.
N1 - Funding Information:
We are very grateful to the Bloomington Drosophila Stock Center, the Vienna Drosophila RNAi Center, and FlyTrap; all are invaluable resources. Catherine Collins, Wesley Grueber, and Dirk Bohmann generously provided us with Drosophila stocks. Tadashi Uemura provided helpful information for imaging da neurons in adults. Michelle Nguyen kindly helped collect flies and embryos. This work was funded by the NIH (R01 GM085115), and M.M.R. was a Pew Scholar in the Biomedical Sciences.
PY - 2014
Y1 - 2014
N2 - Axon injury triggers regeneration through activation of a conserved kinase cascade, which includes the dual leucine zipper kinase (DLK). Although dendrites are damaged during stroke, traumatic brain injury, and seizure, it is not known whether mature neurons monitor dendrite injury and initiate regeneration. We probed the response to dendrite damage using model Drosophila neurons. Two larval neuron types regrew dendrites in distinct ways after all dendrites were removed. Dendrite regeneration was also triggered by injury in adults. Next, we tested whetherdendrite injury was initiated with the same machinery as axon injury. Surprisingly, DLK, JNK, and fos were dispensable for dendrite regeneration. Moreover, this MAP kinase pathway was not activated by injury to dendrites. Thus, neurons respond to dendrite damage and initiate regeneration without using the conserved DLK cascade that triggers axon regeneration.
AB - Axon injury triggers regeneration through activation of a conserved kinase cascade, which includes the dual leucine zipper kinase (DLK). Although dendrites are damaged during stroke, traumatic brain injury, and seizure, it is not known whether mature neurons monitor dendrite injury and initiate regeneration. We probed the response to dendrite damage using model Drosophila neurons. Two larval neuron types regrew dendrites in distinct ways after all dendrites were removed. Dendrite regeneration was also triggered by injury in adults. Next, we tested whetherdendrite injury was initiated with the same machinery as axon injury. Surprisingly, DLK, JNK, and fos were dispensable for dendrite regeneration. Moreover, this MAP kinase pathway was not activated by injury to dendrites. Thus, neurons respond to dendrite damage and initiate regeneration without using the conserved DLK cascade that triggers axon regeneration.
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U2 - 10.1016/j.celrep.2013.12.022
DO - 10.1016/j.celrep.2013.12.022
M3 - Article
C2 - 24412365
AN - SCOPUS:84895753420
SN - 2211-1247
VL - 6
SP - 247
EP - 253
JO - Cell Reports
JF - Cell Reports
IS - 2
ER -