Dendritic patch

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

So far we have discussed patch clamp procedures that target the neuronal soma. However, electrical properties of neurons are critically influenced by dendrites, which not only make up a large portion of neuron’s surface area and but also receive a large proportion of synaptic inputs. The electrical properties of dendritic membrane and somatic membrane can be vey different. Therefore, recording electrical properties directly from dendrites is desired in some experimental designs. Luckily, skilled electrophysiologists have optimized electrophysiological protocols that allow neuronal dendritic recording. Such recordings have provided important information regarding voltage-gated ion channel distribution and function [1-5], differences between somatic and dendritic membrane channel properties [6, 7], and synaptic integration of backpropagating action potentials combined with synaptic potentials [8-10]. The purpose of this chapter is to provide the beginning electrophysiologist with the necessary information required to maximize their success rate for dendritic recordings. While reading this chapter, we also would like to refer the reader to a superb detailed approach to dendritic patching documented by [11].

Original languageEnglish (US)
Title of host publicationNeuromethods
PublisherHumana Press Inc.
Pages225-231
Number of pages7
DOIs
StatePublished - Jan 1 2015

Publication series

NameNeuromethods
Volume112
ISSN (Print)0893-2336
ISSN (Electronic)1940-6045

Fingerprint

Dendrites
Ion Channels
Electric properties
Neurons
Synaptic Potentials
Membranes
Carisoprodol
Action Potentials
Reading
Research Design
Clamping devices
Design of experiments
Electric potential

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)
  • Psychiatry and Mental health

Cite this

Graziane, N., & Dong, Y. (2015). Dendritic patch. In Neuromethods (pp. 225-231). (Neuromethods; Vol. 112). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3274-0_20
Graziane, Nicholas ; Dong, Yan. / Dendritic patch. Neuromethods. Humana Press Inc., 2015. pp. 225-231 (Neuromethods).
@inbook{25fc528ae5fb402683df192f3e4be35b,
title = "Dendritic patch",
abstract = "So far we have discussed patch clamp procedures that target the neuronal soma. However, electrical properties of neurons are critically influenced by dendrites, which not only make up a large portion of neuron’s surface area and but also receive a large proportion of synaptic inputs. The electrical properties of dendritic membrane and somatic membrane can be vey different. Therefore, recording electrical properties directly from dendrites is desired in some experimental designs. Luckily, skilled electrophysiologists have optimized electrophysiological protocols that allow neuronal dendritic recording. Such recordings have provided important information regarding voltage-gated ion channel distribution and function [1-5], differences between somatic and dendritic membrane channel properties [6, 7], and synaptic integration of backpropagating action potentials combined with synaptic potentials [8-10]. The purpose of this chapter is to provide the beginning electrophysiologist with the necessary information required to maximize their success rate for dendritic recordings. While reading this chapter, we also would like to refer the reader to a superb detailed approach to dendritic patching documented by [11].",
author = "Nicholas Graziane and Yan Dong",
year = "2015",
month = "1",
day = "1",
doi = "10.1007/978-1-4939-3274-0_20",
language = "English (US)",
series = "Neuromethods",
publisher = "Humana Press Inc.",
pages = "225--231",
booktitle = "Neuromethods",

}

Graziane, N & Dong, Y 2015, Dendritic patch. in Neuromethods. Neuromethods, vol. 112, Humana Press Inc., pp. 225-231. https://doi.org/10.1007/978-1-4939-3274-0_20

Dendritic patch. / Graziane, Nicholas; Dong, Yan.

Neuromethods. Humana Press Inc., 2015. p. 225-231 (Neuromethods; Vol. 112).

Research output: Chapter in Book/Report/Conference proceedingChapter

TY - CHAP

T1 - Dendritic patch

AU - Graziane, Nicholas

AU - Dong, Yan

PY - 2015/1/1

Y1 - 2015/1/1

N2 - So far we have discussed patch clamp procedures that target the neuronal soma. However, electrical properties of neurons are critically influenced by dendrites, which not only make up a large portion of neuron’s surface area and but also receive a large proportion of synaptic inputs. The electrical properties of dendritic membrane and somatic membrane can be vey different. Therefore, recording electrical properties directly from dendrites is desired in some experimental designs. Luckily, skilled electrophysiologists have optimized electrophysiological protocols that allow neuronal dendritic recording. Such recordings have provided important information regarding voltage-gated ion channel distribution and function [1-5], differences between somatic and dendritic membrane channel properties [6, 7], and synaptic integration of backpropagating action potentials combined with synaptic potentials [8-10]. The purpose of this chapter is to provide the beginning electrophysiologist with the necessary information required to maximize their success rate for dendritic recordings. While reading this chapter, we also would like to refer the reader to a superb detailed approach to dendritic patching documented by [11].

AB - So far we have discussed patch clamp procedures that target the neuronal soma. However, electrical properties of neurons are critically influenced by dendrites, which not only make up a large portion of neuron’s surface area and but also receive a large proportion of synaptic inputs. The electrical properties of dendritic membrane and somatic membrane can be vey different. Therefore, recording electrical properties directly from dendrites is desired in some experimental designs. Luckily, skilled electrophysiologists have optimized electrophysiological protocols that allow neuronal dendritic recording. Such recordings have provided important information regarding voltage-gated ion channel distribution and function [1-5], differences between somatic and dendritic membrane channel properties [6, 7], and synaptic integration of backpropagating action potentials combined with synaptic potentials [8-10]. The purpose of this chapter is to provide the beginning electrophysiologist with the necessary information required to maximize their success rate for dendritic recordings. While reading this chapter, we also would like to refer the reader to a superb detailed approach to dendritic patching documented by [11].

UR - http://www.scopus.com/inward/record.url?scp=84950311437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84950311437&partnerID=8YFLogxK

U2 - 10.1007/978-1-4939-3274-0_20

DO - 10.1007/978-1-4939-3274-0_20

M3 - Chapter

T3 - Neuromethods

SP - 225

EP - 231

BT - Neuromethods

PB - Humana Press Inc.

ER -

Graziane N, Dong Y. Dendritic patch. In Neuromethods. Humana Press Inc. 2015. p. 225-231. (Neuromethods). https://doi.org/10.1007/978-1-4939-3274-0_20