Density-based diamagnetic separation

Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions

Adam Winkleman, Raquel Perez-Castillejos, Katherine L. Gudiksen, Scott T. Phillips, Mara Prentiss, George M. Whitesides

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

This paper describes the fabrication of a fluidic device for detecting and separating diamagnetic materials that differ in density. The basis for the separation is the balance of the magnetic and gravitational forces on diamagnetic materials suspended in a paramagnetic medium. The paper demonstrates two applications of separations involving particles suspended in static fluids for detecting the following: (i) the binding of streptavidin to solid-supported biotin and (ii) the binding of citrate-capped gold nanoparticles to amine-modified polystyrene spheres. The paper also demonstrates a microfluidic device in which polystyrene particles that differ in their content of CH 2Cl groups are continuously separated and collected in a flowing stream of an aqueous solution of GdCl3. The procedures for separation and detection described in this paper require only gadolinium salts, two NdFeB magnets, and simple microfluidic devices fabricated from poly-(dimethylsiloxane) . This device requires no power, has no moving parts, and may be suitable for use in resource-poor environments.

Original languageEnglish (US)
Pages (from-to)6542-6550
Number of pages9
JournalAnalytical Chemistry
Volume79
Issue number17
DOIs
StatePublished - Sep 1 2007

Fingerprint

Diamagnetic materials
Polystyrenes
Microfluidics
Fluidic devices
Streptavidin
Gadolinium
Biotin
Citric Acid
Gold
Amines
Magnets
Salts
Nanoparticles
Fabrication
Fluids

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

Cite this

Winkleman, A., Perez-Castillejos, R., Gudiksen, K. L., Phillips, S. T., Prentiss, M., & Whitesides, G. M. (2007). Density-based diamagnetic separation: Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions. Analytical Chemistry, 79(17), 6542-6550. https://doi.org/10.1021/ac070500b
Winkleman, Adam ; Perez-Castillejos, Raquel ; Gudiksen, Katherine L. ; Phillips, Scott T. ; Prentiss, Mara ; Whitesides, George M. / Density-based diamagnetic separation : Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions. In: Analytical Chemistry. 2007 ; Vol. 79, No. 17. pp. 6542-6550.
@article{047a86240a5145e9b6a268777d124dc6,
title = "Density-based diamagnetic separation: Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions",
abstract = "This paper describes the fabrication of a fluidic device for detecting and separating diamagnetic materials that differ in density. The basis for the separation is the balance of the magnetic and gravitational forces on diamagnetic materials suspended in a paramagnetic medium. The paper demonstrates two applications of separations involving particles suspended in static fluids for detecting the following: (i) the binding of streptavidin to solid-supported biotin and (ii) the binding of citrate-capped gold nanoparticles to amine-modified polystyrene spheres. The paper also demonstrates a microfluidic device in which polystyrene particles that differ in their content of CH 2Cl groups are continuously separated and collected in a flowing stream of an aqueous solution of GdCl3. The procedures for separation and detection described in this paper require only gadolinium salts, two NdFeB magnets, and simple microfluidic devices fabricated from poly-(dimethylsiloxane) . This device requires no power, has no moving parts, and may be suitable for use in resource-poor environments.",
author = "Adam Winkleman and Raquel Perez-Castillejos and Gudiksen, {Katherine L.} and Phillips, {Scott T.} and Mara Prentiss and Whitesides, {George M.}",
year = "2007",
month = "9",
day = "1",
doi = "10.1021/ac070500b",
language = "English (US)",
volume = "79",
pages = "6542--6550",
journal = "Analytical Chemistry",
issn = "0003-2700",
publisher = "American Chemical Society",
number = "17",

}

Winkleman, A, Perez-Castillejos, R, Gudiksen, KL, Phillips, ST, Prentiss, M & Whitesides, GM 2007, 'Density-based diamagnetic separation: Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions', Analytical Chemistry, vol. 79, no. 17, pp. 6542-6550. https://doi.org/10.1021/ac070500b

Density-based diamagnetic separation : Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions. / Winkleman, Adam; Perez-Castillejos, Raquel; Gudiksen, Katherine L.; Phillips, Scott T.; Prentiss, Mara; Whitesides, George M.

In: Analytical Chemistry, Vol. 79, No. 17, 01.09.2007, p. 6542-6550.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Density-based diamagnetic separation

T2 - Devices for detecting binding events and for collecting unlabeled diamagnetic particles in paramagnetic solutions

AU - Winkleman, Adam

AU - Perez-Castillejos, Raquel

AU - Gudiksen, Katherine L.

AU - Phillips, Scott T.

AU - Prentiss, Mara

AU - Whitesides, George M.

PY - 2007/9/1

Y1 - 2007/9/1

N2 - This paper describes the fabrication of a fluidic device for detecting and separating diamagnetic materials that differ in density. The basis for the separation is the balance of the magnetic and gravitational forces on diamagnetic materials suspended in a paramagnetic medium. The paper demonstrates two applications of separations involving particles suspended in static fluids for detecting the following: (i) the binding of streptavidin to solid-supported biotin and (ii) the binding of citrate-capped gold nanoparticles to amine-modified polystyrene spheres. The paper also demonstrates a microfluidic device in which polystyrene particles that differ in their content of CH 2Cl groups are continuously separated and collected in a flowing stream of an aqueous solution of GdCl3. The procedures for separation and detection described in this paper require only gadolinium salts, two NdFeB magnets, and simple microfluidic devices fabricated from poly-(dimethylsiloxane) . This device requires no power, has no moving parts, and may be suitable for use in resource-poor environments.

AB - This paper describes the fabrication of a fluidic device for detecting and separating diamagnetic materials that differ in density. The basis for the separation is the balance of the magnetic and gravitational forces on diamagnetic materials suspended in a paramagnetic medium. The paper demonstrates two applications of separations involving particles suspended in static fluids for detecting the following: (i) the binding of streptavidin to solid-supported biotin and (ii) the binding of citrate-capped gold nanoparticles to amine-modified polystyrene spheres. The paper also demonstrates a microfluidic device in which polystyrene particles that differ in their content of CH 2Cl groups are continuously separated and collected in a flowing stream of an aqueous solution of GdCl3. The procedures for separation and detection described in this paper require only gadolinium salts, two NdFeB magnets, and simple microfluidic devices fabricated from poly-(dimethylsiloxane) . This device requires no power, has no moving parts, and may be suitable for use in resource-poor environments.

UR - http://www.scopus.com/inward/record.url?scp=34548639255&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=34548639255&partnerID=8YFLogxK

U2 - 10.1021/ac070500b

DO - 10.1021/ac070500b

M3 - Article

VL - 79

SP - 6542

EP - 6550

JO - Analytical Chemistry

JF - Analytical Chemistry

SN - 0003-2700

IS - 17

ER -