TY - JOUR
T1 - Density‐Dependent Changes in Gangliosides and Sialidase Activity of Murine Neuroblastoma Cells
AU - Schengrund, C. ‐L
AU - Repman, M. A.
PY - 1982/10
Y1 - 1982/10
N2 - Abstract: Density‐dependent changes in ganglioside composition, Vibrio cholerae neuraminidase (VCN)‐susceptible sialyl residues, and membrane‐ associated sialidase activity were determined for the cholinergic murine neuroblastoma cell line S20Y. A decrease in total ganglioside sialic acid and VCN‐releasable sialic acid was observed with increasing cell density. GM3 was the major ganglioside component of preconfluent S20Y cells, whereas GDIA was predominant in postconfluent cells. Sialidase activity increased in confluent and postconfluent cells and may account for the reduction in total ganglioside sialic acid observed with increasing cell density. In contrast, while adrenergic N115 cells showed a decrease in VCN‐susceptible sialic acid residues with increasing cell density, there was no significant change in ganglioside composition or ganglioside sialic acid levels.
AB - Abstract: Density‐dependent changes in ganglioside composition, Vibrio cholerae neuraminidase (VCN)‐susceptible sialyl residues, and membrane‐ associated sialidase activity were determined for the cholinergic murine neuroblastoma cell line S20Y. A decrease in total ganglioside sialic acid and VCN‐releasable sialic acid was observed with increasing cell density. GM3 was the major ganglioside component of preconfluent S20Y cells, whereas GDIA was predominant in postconfluent cells. Sialidase activity increased in confluent and postconfluent cells and may account for the reduction in total ganglioside sialic acid observed with increasing cell density. In contrast, while adrenergic N115 cells showed a decrease in VCN‐susceptible sialic acid residues with increasing cell density, there was no significant change in ganglioside composition or ganglioside sialic acid levels.
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U2 - 10.1111/j.1471-4159.1982.tb11480.x
DO - 10.1111/j.1471-4159.1982.tb11480.x
M3 - Article
C2 - 7119793
AN - SCOPUS:0020364635
VL - 39
SP - 940
EP - 947
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
SN - 0022-3042
IS - 4
ER -