Density‐Dependent Changes in Gangliosides and Sialidase Activity of Murine Neuroblastoma Cells

C. ‐L Schengrund, M. A. Repman

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Abstract: Density‐dependent changes in ganglioside composition, Vibrio cholerae neuraminidase (VCN)‐susceptible sialyl residues, and membrane‐ associated sialidase activity were determined for the cholinergic murine neuroblastoma cell line S20Y. A decrease in total ganglioside sialic acid and VCN‐releasable sialic acid was observed with increasing cell density. GM3 was the major ganglioside component of preconfluent S20Y cells, whereas GDIA was predominant in postconfluent cells. Sialidase activity increased in confluent and postconfluent cells and may account for the reduction in total ganglioside sialic acid observed with increasing cell density. In contrast, while adrenergic N115 cells showed a decrease in VCN‐susceptible sialic acid residues with increasing cell density, there was no significant change in ganglioside composition or ganglioside sialic acid levels.

Original languageEnglish (US)
Pages (from-to)940-947
Number of pages8
JournalJournal of neurochemistry
Volume39
Issue number4
DOIs
StatePublished - Oct 1982

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Cellular and Molecular Neuroscience

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