Depletion of abundant proteins from non-human primate serum for biomarker studies

Willard M. Freeman, Melinda E. Lull, Michael T. Guilford, Kent E. Vrana

Research output: Contribution to journalArticle

17 Scopus citations

Abstract

Non-human primates are an important biomedical research model organism and offer great promise for serum biomarker proteomic studies. However, potential obstacles to these studies include affinity serum depletion methods based on human antigens, depletion methods altering quantitation, and incomplete non-human primate genome sequences for protein identification. In the present study, high-abundance protein removal from monkey serum using a human multiple affinity removal system (MARS) was shown to be specific and did not alter quantitation. Depleted serum also demonstrated greater sensitivity for previously masked, lower-abundance proteins.

Original languageEnglish (US)
Pages (from-to)3109-3113
Number of pages5
JournalProteomics
Volume6
Issue number10
DOIs
StatePublished - May 2006

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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