Description and targeted deletion of 5' hypersensitive site 5 and 6 of the mouse β-globin locus control region

M. A. Bender, Andreas Reik, Jennie Close, Agnes Telling, Elliot Epner, Steven Fiering, Ross Hardison, Mark Groudine

Research output: Contribution to journalArticlepeer-review

68 Scopus citations

Abstract

The most upstream hypersensitive site (HS) of the β-globin locus control region (LCR) in humans (5' HS 5) and chickens (5' HS 4) can act as an insulating element in some gain of function assays and may demarcate a β- globin domain. We have mapped the most upstream HSs of the mouse β-globin LCR and sequenced this region. We find that mice have a region homologous to human 5' HS 5 that is associated with a minor HS. In addition we map a unique HS upstream of 5' HS 5 and refer to this novel site as mouse 5' HS 6. We have also generated mice containing a targeted deletion of the region containing 5' HS 5 and 6. We find that after excision of the selectable marker in vivo, deletion of 5' HS 5 and 6 has a minimal effect on transcription and does not prevent formation of the remaining LCR HSs. Taken together these findings suggest that the most upstream HSs of the mouse β-globin LCR are not necessary for maintaining the β-globin locus in an active configuration or to protect it from a surrounding repressive chromatin environment.

Original languageEnglish (US)
Pages (from-to)4394-4403
Number of pages10
JournalBlood
Volume92
Issue number11
DOIs
StatePublished - Dec 1 1998

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Immunology
  • Hematology
  • Cell Biology

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