This chapter describes procedural aspects for development of ribozymes in general, and specifically, that cleave mRNA to an essential cellular gene, the AC40 subunit of RNA pol I. Ribozyme design includes functional selection of binding sites followed by computer modeling. These ribozymes are being used in vectors that target expression to the prostate via tissue specific promoters (Voeks, Norris, and Clawson, 1998) and have demonstrated efficacy.
|Original language||English (US)|
|Number of pages||9|
|Journal||Advances in experimental medicine and biology|
|State||Published - Jan 1 2000|
All Science Journal Classification (ASJC) codes
- Biochemistry, Genetics and Molecular Biology(all)