Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies

Jordan Leyton-Mange, Sung Yang, Meghan H. Hoskins, Robert F. Kunz, Jeffrey D. Zahn, Cheng Dong

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Experimental models that mimic the flow conditions in microcapillaries have suggested that the local shear stresses and shear rates can mediate tumor cell and leukocyte arrest on the endothelium and subsequent sustained adhesion. However, further investigation has been limited by the lack of experimental models that allow quantitative measurement of the hydrodynamic environment over adherent cells. The purpose of this study was to develop a system capable of acquiring quantitative flow profiles over adherent cells. By combining the techniques of side-view imaging and particle image velocimetry (PIV), an in vitro model was con" structed that is capable of obtaining quantitative flow data over cells adhering to the endothelium. The velocity over an adherent leukocyte was measured and the shear rate was calculated under low and high upstream wall shear. The microcapillary channel was modeled using computational fluid dynamics (CFD) and the calculated velocity profiles over cells under the low and high shear rates were compared to experimental results. The drag force applied to each cell by the fluid was then computed. This system provides a means for future study of the forces underlying adhesion by permitting characterization of the local hydrodynamic conditions over adherent cells.

Original languageEnglish (US)
Pages (from-to)271-278
Number of pages8
JournalJournal of Biomechanical Engineering
Volume128
Issue number2
DOIs
StatePublished - Apr 1 2006

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Rheology
Cell Adhesion
Velocity measurement
Shear deformation
Adhesion
Imaging techniques
Substrates
Hydrodynamics
Shear walls
Drag
Shear stress
Tumors
Computational fluid dynamics
Endothelium
Cells
Leukocytes
Theoretical Models
Fluids
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Physiology (medical)

Cite this

Leyton-Mange, Jordan ; Yang, Sung ; Hoskins, Meghan H. ; Kunz, Robert F. ; Zahn, Jeffrey D. ; Dong, Cheng. / Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies. In: Journal of Biomechanical Engineering. 2006 ; Vol. 128, No. 2. pp. 271-278.
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Design of a side-view particle imaging velocimetry flow system for cell-substrate adhesion studies. / Leyton-Mange, Jordan; Yang, Sung; Hoskins, Meghan H.; Kunz, Robert F.; Zahn, Jeffrey D.; Dong, Cheng.

In: Journal of Biomechanical Engineering, Vol. 128, No. 2, 01.04.2006, p. 271-278.

Research output: Contribution to journalArticle

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